TY - CHAP
T1 - Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II
T2 - Advanced Characterization
AU - Bitzenhofer, Nora Lisa
AU - Hilgers, Fabienne
AU - Bosio, Gabriela N.
AU - Torra, Joaquim
AU - Casini, Giorgia
AU - Beinlich, Felix R.M.
AU - Knieps-Grünhagen, Esther
AU - Gordeliy, Valentin
AU - Jaeger, Karl Erich
AU - Nonell, Santi
AU - Krauss, Ulrich
AU - Gensch, Thomas
AU - Drepper, Thomas
N1 - Funding Information:
N.L.B., F.H., G.B., and J.T. contributed equally to the preparation of the chapter. S.N., U.K., T.G., and T.D. are co-corresponding authors. The PS characterization was supported by grants from the Bioeconomy Science Center awarded to F.H. The scientific activities of the Bioeconomy Science Center were financially supported by the Ministry of Innovation, Science and Research of the German federal state of North Rhine-Westphalia MIWF within the frame-work of the NRW Strategieprojekt BioSC (No. 313/323-400-00213). The authors thank Ivan Gushchin for his contributions to writing and editing of this chapter.
Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
AB - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.
KW - Flavin
KW - Flavin-binding fluorescent protein
KW - Fluorescence brightness
KW - Fluorescence lifetime
KW - Fluorescence microscopy
KW - Genetically encoded photosensitizer
KW - LOV domain
KW - Phototoxicity
UR - http://www.scopus.com/inward/record.url?scp=85137880361&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2667-2_7
DO - 10.1007/978-1-0716-2667-2_7
M3 - Chapter
C2 - 36107341
AN - SCOPUS:85137880361
T3 - Methods in Molecular Biology
SP - 143
EP - 183
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -