Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer; Fabienne Hilgers; Gabriela N. Bosio; Joaquim Torra; Giorgia Casini; Felix R.M. Beinlich; Esther Knieps-Grünhagen; Valentin Gordeliy; Karl Erich Jaeger; Santi Nonell; Ulrich Krauss; Thomas Gensch; Thomas Drepper

doi:10.1007/978-1-0716-2667-2_7

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, Thomas Drepper

Producció científica: Capítol de llibre › Capítol › Avaluat per experts

3 Cites (Scopus)

Resum

Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

Idioma original	Anglès
Títol de la publicació	Methods in Molecular Biology
Editor	Humana Press Inc.
Pàgines	143-183
Nombre de pàgines	41
ISBN (electrònic)	978-1-0716-2667-2
ISBN (imprès)	978-1-0716-2666-5
DOIs	https://doi.org/10.1007/978-1-0716-2667-2_7
Estat de la publicació	Publicada - de set. 2023

Sèrie de publicacions

Nom	Methods in Molecular Biology
Volum	2564
ISSN (imprès)	1064-3745
ISSN (electrònic)	1940-6029

Accés al document

10.1007/978-1-0716-2667-2_7

Altres arxius i enllaços

Enllaç a la publicació de Scopus

Com citar-ho

Bitzenhofer, N. L., Hilgers, F., Bosio, G. N., Torra, J., Casini, G., Beinlich, F. R. M., Knieps-Grünhagen, E., Gordeliy, V., Jaeger, K. E., Nonell, S., Krauss, U., Gensch, T., & Drepper, T. (2023). Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. In Methods in Molecular Biology (pàg. 143-183). (Methods in Molecular Biology; Vol. 2564). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-2667-2_7

@inbook{24fe8a07d3a046f4a3f11ee69e1f216d,

title = "Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization",

abstract = "Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.",

keywords = "Flavin, Flavin-binding fluorescent protein, Fluorescence brightness, Fluorescence lifetime, Fluorescence microscopy, Genetically encoded photosensitizer, LOV domain, Phototoxicity",

author = "Bitzenhofer, {Nora Lisa} and Fabienne Hilgers and Bosio, {Gabriela N.} and Joaquim Torra and Giorgia Casini and Beinlich, {Felix R.M.} and Esther Knieps-Gr{\"u}nhagen and Valentin Gordeliy and Jaeger, {Karl Erich} and Santi Nonell and Ulrich Krauss and Thomas Gensch and Thomas Drepper",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2023",

month = sep,

doi = "10.1007/978-1-0716-2667-2_7",

language = "English",

isbn = "978-1-0716-2666-5",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "143--183",

booktitle = "Methods in Molecular Biology",

}

Bitzenhofer, NL, Hilgers, F, Bosio, GN, Torra, J, Casini, G, Beinlich, FRM, Knieps-Grünhagen, E, Gordeliy, V, Jaeger, KE, Nonell, S, Krauss, U, Gensch, T & Drepper, T 2023, Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2564, Humana Press Inc., pàg. 143-183. https://doi.org/10.1007/978-1-0716-2667-2_7

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. / Bitzenhofer, Nora Lisa; Hilgers, Fabienne; Bosio, Gabriela N. et al.
Methods in Molecular Biology. Humana Press Inc., 2023. pàg. 143-183 (Methods in Molecular Biology; Vol. 2564).

Producció científica: Capítol de llibre › Capítol › Avaluat per experts

TY - CHAP

T1 - Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II

T2 - Advanced Characterization

AU - Bitzenhofer, Nora Lisa

AU - Hilgers, Fabienne

AU - Bosio, Gabriela N.

AU - Torra, Joaquim

AU - Casini, Giorgia

AU - Beinlich, Felix R.M.

AU - Knieps-Grünhagen, Esther

AU - Gordeliy, Valentin

AU - Jaeger, Karl Erich

AU - Nonell, Santi

AU - Krauss, Ulrich

AU - Gensch, Thomas

AU - Drepper, Thomas

PY - 2023/9

Y1 - 2023/9

N2 - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

AB - Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

KW - Flavin

KW - Flavin-binding fluorescent protein

KW - Fluorescence brightness

KW - Fluorescence lifetime

KW - Fluorescence microscopy

KW - Genetically encoded photosensitizer

KW - LOV domain

KW - Phototoxicity

UR - http://www.scopus.com/inward/record.url?scp=85137880361&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2667-2_7

DO - 10.1007/978-1-0716-2667-2_7

M3 - Chapter

C2 - 36107341

AN - SCOPUS:85137880361

SN - 978-1-0716-2666-5

T3 - Methods in Molecular Biology

SP - 143

EP - 183

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Resum

Sèrie de publicacions

Accés al document

Altres arxius i enllaços

Fingerprint

Com citar-ho