Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer; Fabienne Hilgers; Gabriela N. Bosio; Joaquim Torra; Giorgia Casini; Felix R.M. Beinlich; Esther Knieps-Grünhagen; Valentin Gordeliy; Karl Erich Jaeger; Santi Nonell; Ulrich Krauss; Thomas Gensch; Thomas Drepper

doi:10.1007/978-1-0716-2667-2_7

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl Erich Jaeger, Santi Nonell, Ulrich Krauss, Thomas Gensch, Thomas Drepper

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3 Citas (Scopus)

Resumen

Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

Idioma original	Inglés
Título de la publicación alojada	Methods in Molecular Biology
Editorial	Humana Press Inc.
Páginas	143-183
Número de páginas	41
ISBN (versión digital)	978-1-0716-2667-2
ISBN (versión impresa)	978-1-0716-2666-5
DOI	https://doi.org/10.1007/978-1-0716-2667-2_7
Estado	Publicada - 2023

Serie de la publicación

Nombre	Methods in Molecular Biology
Volumen	2564
ISSN (versión impresa)	1064-3745
ISSN (versión digital)	1940-6029

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Bitzenhofer, N. L., Hilgers, F., Bosio, G. N., Torra, J., Casini, G., Beinlich, F. R. M., Knieps-Grünhagen, E., Gordeliy, V., Jaeger, K. E., Nonell, S., Krauss, U., Gensch, T., & Drepper, T. (2023). Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. En Methods in Molecular Biology (pp. 143-183). (Methods in Molecular Biology; Vol. 2564). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-2667-2_7

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abstract = "Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.",

keywords = "Flavin, Flavin-binding fluorescent protein, Fluorescence brightness, Fluorescence lifetime, Fluorescence microscopy, Genetically encoded photosensitizer, LOV domain, Phototoxicity",

author = "Bitzenhofer, {Nora Lisa} and Fabienne Hilgers and Bosio, {Gabriela N.} and Joaquim Torra and Giorgia Casini and Beinlich, {Felix R.M.} and Esther Knieps-Gr{\"u}nhagen and Valentin Gordeliy and Jaeger, {Karl Erich} and Santi Nonell and Ulrich Krauss and Thomas Gensch and Thomas Drepper",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2023",

doi = "10.1007/978-1-0716-2667-2_7",

language = "English",

isbn = "978-1-0716-2666-5",

series = "Methods in Molecular Biology",

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Bitzenhofer, NL, Hilgers, F, Bosio, GN, Torra, J, Casini, G, Beinlich, FRM, Knieps-Grünhagen, E, Gordeliy, V, Jaeger, KE, Nonell, S, Krauss, U, Gensch, T & Drepper, T 2023, Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. En Methods in Molecular Biology. Methods in Molecular Biology, vol. 2564, Humana Press Inc., pp. 143-183. https://doi.org/10.1007/978-1-0716-2667-2_7

Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization. / Bitzenhofer, Nora Lisa; Hilgers, Fabienne; Bosio, Gabriela N. et al.
Methods in Molecular Biology. Humana Press Inc., 2023. p. 143-183 (Methods in Molecular Biology; Vol. 2564).

Producción científica: Capítulo del libro › Capítulo › revisión exhaustiva

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T1 - Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II

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AU - Bitzenhofer, Nora Lisa

AU - Hilgers, Fabienne

AU - Bosio, Gabriela N.

AU - Torra, Joaquim

AU - Casini, Giorgia

AU - Beinlich, Felix R.M.

AU - Knieps-Grünhagen, Esther

AU - Gordeliy, Valentin

AU - Jaeger, Karl Erich

AU - Nonell, Santi

AU - Krauss, Ulrich

AU - Gensch, Thomas

AU - Drepper, Thomas

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KW - Flavin-binding fluorescent protein

KW - Fluorescence brightness

KW - Fluorescence lifetime

KW - Fluorescence microscopy

KW - Genetically encoded photosensitizer

KW - LOV domain

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Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

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