Development and Characterization of Flavin-Binding Fluorescent Proteins, Part II: Advanced Characterization

Nora Lisa Bitzenhofer, Fabienne Hilgers, Gabriela N. Bosio, Joaquim Torra, Giorgia Casini, Felix R.M. Beinlich, Esther Knieps-Grünhagen, Valentin Gordeliy, Karl Erich Jaeger, Santi Nonell, Ulrich Krauss*, Thomas Gensch, Thomas Drepper

*Corresponding author for this work

Research output: Book chapterChapterpeer-review

3 Citations (Scopus)

Abstract

Flavin-based fluorescent proteins (FbFPs), a class of small fluorescent proteins derived from light-oxygen-voltage (LOV) domains, bind ubiquitous endogenous flavins as chromophores. Due to their unique properties, they can be used as versatile in vivo reporter proteins under aerobic and anaerobic conditions. This chapter presents methodologies for in-depth characterization of the biochemical, spectroscopic, photophysical, and photochemical properties of FbFPs.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-183
Number of pages41
ISBN (Electronic)978-1-0716-2667-2
ISBN (Print)978-1-0716-2666-5
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2564
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Flavin
  • Flavin-binding fluorescent protein
  • Fluorescence brightness
  • Fluorescence lifetime
  • Fluorescence microscopy
  • Genetically encoded photosensitizer
  • LOV domain
  • Phototoxicity

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