TY - JOUR
T1 - Spatial Control of Neuronal Adhesion on Diamond-Like Carbon
AU - Dugan, James M.
AU - Colominas, Carles
AU - Garcia-Granada, Andrés Amador
AU - Claeyssens, Frederik
N1 - Funding Information:
The authors are grateful to EPSRC for funding this work in the form of a PDRA position for JMD supported under grant number EP/ K002503/1. We also acknowledge funding from the EPSRC (Grant No. EP/I007695/1) and the Medical Research Council (MR/ L012669/1) for establishing the laser laboratory. The Sheffield Surface Analysis Centre and Kroto Imaging Facility are also gratefully acknowledged.
Publisher Copyright:
Copyright © 2021 Dugan, Colominas, Garcia-Granada and Claeyssens.
PY - 2021/11/22
Y1 - 2021/11/22
N2 - This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.
AB - This study reports a route to spatial control of neuronal adhesion onto Diamond-Like Carbon (DLC) by surface functionalisation by poly (oligo-ethyleneglycol methacrylate) (pOEGMA) and consequent laser ablation to produce cell adhesive tracks. DLC can be deposited as a tough and low friction coating on implantable devices and surgical instruments and has favourable properties for use as a biomaterial. The pOEGMA surface coating renders the DLC surface antifouling and the laser ablation creates graphitised tracks on the surface. The surfaces were coated with laminin, which adhered preferentially to the ablation tracks. The patterned surfaces were investigated for neuronal cell growth with NG108-15 cells for short term culture and rat neural stem cells for longer term culture. The cells initially adhered highly selectively to the ablation tracks while longer term cell culture revealed a more uniform cell coverage of the surface.
KW - atom transfer radical polymerization
KW - diamond-like carbon
KW - laser ablation
KW - neuronal cell culture
KW - poly(oligo (ethylene glycol) methacrylate)
UR - http://www.scopus.com/inward/record.url?scp=85120703829&partnerID=8YFLogxK
U2 - 10.3389/fmats.2021.756055
DO - 10.3389/fmats.2021.756055
M3 - Article
AN - SCOPUS:85120703829
SN - 2296-8016
VL - 8
JO - Frontiers in Materials
JF - Frontiers in Materials
M1 - 756055
ER -