TY - JOUR
T1 - NanoSOSG
T2 - A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
AU - Ruiz-González, Rubén
AU - Bresolí-Obach, Roger
AU - Gulías, Òscar
AU - Agut, Montserrat
AU - Savoie, Huguette
AU - Boyle, Ross W.
AU - Nonell, Santi
AU - Giuntini, Francesca
N1 - Funding Information:
Financial support for this research was obtained from the Spanish Ministerio de Economía y Competitividad (CTQ2010-20870-C03-01 and CTQ2013-48767-C3-1-R). R.B-O thank the Generalitat de Catalunya (DURSI) and the European Social Fund for predoctoral fellowships (2015 FI_B 00315). R.R.-G. thanks the Spanish Ministerio de Educación Cultura y Deporte for a “Beca de movilidad para estudiantes en programas de Doctorado con Mención hacia la excelencia” fellowship. OG thanks IQS for his predoctoral fellowship. FG and RWB thank EPSRC for funding (EP/H000151/1).
Funding Information:
Financial support for this research was obtained from the Spanish Ministerio de Econom?a y Competitividad (CTQ2010-20870-C03-01 and CTQ2013-48767-C3-1-R). R.B-O thank the Generalitat de Catalunya (DURSI) and the European Social Fund for predoctoral fellowships (2015 FI_B 00315). R.R.-G. thanks the Spanish Ministerio de Educaci?n Cultura y Deporte for a ?Beca de movilidad para estudiantes en programas de Doctorado con Menci?n hacia la excelencia? fellowship. OG thanks IQS for his predoctoral fellowship. FG and RWB thank EPSRC for funding (EP/H000151/1).
Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2017/3/6
Y1 - 2017/3/6
N2 - A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1O2 with an increase in fluorescence.
AB - A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1O2 with an increase in fluorescence.
KW - fluorescent probes
KW - intracellular sensors
KW - nanoparticles
KW - optical sensors
KW - singlet oxygen
UR - http://www.scopus.com/inward/record.url?scp=85011712765&partnerID=8YFLogxK
U2 - 10.1002/anie.201609050
DO - 10.1002/anie.201609050
M3 - Article
C2 - 28151569
AN - SCOPUS:85011712765
SN - 1433-7851
VL - 56
SP - 2885
EP - 2888
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 11
ER -