NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen

Rubén Ruiz-González, Roger Bresolí-Obach, Òscar Gulías, Montserrat Agut, Huguette Savoie, Ross W. Boyle, Santi Nonell, Francesca Giuntini

Research output: Indexed journal article Articlepeer-review

67 Citations (Scopus)

Abstract

A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1O2 with an increase in fluorescence.

Original languageEnglish
Pages (from-to)2885-2888
Number of pages4
JournalAngewandte Chemie - International Edition
Volume56
Issue number11
DOIs
Publication statusPublished - 6 Mar 2017

Keywords

  • fluorescent probes
  • intracellular sensors
  • nanoparticles
  • optical sensors
  • singlet oxygen

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