NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen

Rubén Ruiz-González; Roger Bresolí-Obach; Òscar Gulías; Montserrat Agut; Huguette Savoie; Ross W. Boyle; Santi Nonell; Francesca Giuntini

doi:10.1002/anie.201609050

NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen

Rubén Ruiz-González, Roger Bresolí-Obach, Òscar Gulías, Montserrat Agut, Huguette Savoie, Ross W. Boyle^*, Santi Nonell, Francesca Giuntini

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

77 Citations (Scopus)

Abstract

A biocompatible fluorescent nanoprobe for singlet oxygen (¹O₂) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green^® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to ¹O₂. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated ¹O₂ with an increase in fluorescence.

Original language	English
Pages (from-to)	2885-2888
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	56
Issue number	11
DOIs	https://doi.org/10.1002/anie.201609050
Publication status	Published - 6 Mar 2017

Keywords

fluorescent probes
intracellular sensors
nanoparticles
optical sensors
singlet oxygen

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title = "NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen",

abstract = "A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green{\textregistered} (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1O2 with an increase in fluorescence.",

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AU - Ruiz-González, Rubén

AU - Bresolí-Obach, Roger

AU - Gulías, Òscar

AU - Agut, Montserrat

AU - Savoie, Huguette

AU - Boyle, Ross W.

AU - Nonell, Santi

AU - Giuntini, Francesca

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AB - A biocompatible fluorescent nanoprobe for singlet oxygen (1O2) detection in biological systems was designed, synthesized, and characterized, that circumvents many of the limitations of the molecular probe Singlet Oxygen Sensor Green® (SOSG). This widely used commercial singlet oxygen probe was covalently linked to a polyacrylamide nanoparticle core using different architectures to optimize the response to 1O2. In contrast to its molecular counterpart, the optimum SOSG-based nanoprobe, which we call NanoSOSG, is readily internalized by E. coli cells and does not interact with bovine serum albumin. Furthermore, the spectral characteristics do not change inside cells, and the probe responds to intracellularly generated 1O2 with an increase in fluorescence.

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NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen

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