TY - JOUR
T1 - Evaluating the alterations of the estrogen-responsive genes in Cyprinodon variegatus larvae for biomonitoring the impacts of estrogenic endocrine disruptors (EEDs)
AU - Abril, Sandra Isabel Moreno
AU - Pin, Ana Olmos
AU - Schonemann, Alexandre M.
AU - Bellot, Marina
AU - Gómez-Canela, Cristian
AU - Beiras, Ricardo
N1 - Funding Information:
This work was co-funded by the European Union through the European Regional Development Fund (ERDF), the GLAUKOS Project (this project has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 887711 . The JU receives support from the European Union’s Horizon 2020 research and innovation programme and the Bio-based Industries Consortium) and the LABPLAS Project (EU Horizon 2020 research and innovation programme under grant agreement No. 101003954 ). Special thanks to the funding for open access charge go to Universidade de Vigo/CISUG. The ECOTOX group receives funding from the Marine Research Center (CIM) of the University of Vigo .
Funding Information:
This work was co-funded by the European Union through the European Regional Development Fund (ERDF), the GLAUKOS Project (this project has received funding from the Bio-based Industries Joint Undertaking (JU) under the European Union's Horizon 2020 research and innovation programme under grant agreement No. 887711. The JU receives support from the European Union's Horizon 2020 research and innovation programme and the Bio-based Industries Consortium) and the LABPLAS Project (EU Horizon 2020 research and innovation programme under grant agreement No. 101003954). Special thanks to the funding for open access charge go to Universidade de Vigo/CISUG. The ECOTOX group receives funding from the Marine Research Center (CIM) of the University of Vigo.
Publisher Copyright:
© 2022 The Authors
PY - 2023/1
Y1 - 2023/1
N2 - Currently, endocrine disruptors (EDs) can be found in all the environmental compartments. To understand the effects of estrogenic EDs (EEDs), adults of Cyprinodon variegatus have been classically used as a marine model. However, it is during development that exposure to contaminants may generate permanent consequences. Thus, the aim of this study was to verify the effects produced by acute exposure to 17α-ethinylestradiol (EE2) in C. variegatus larvae. Quantitative PCR (qPCR) results revealed the induction of vtg and zp gene expression on exposure to 1000 ng/L EE2 and the induction of vtgc, zp2, zp3 and cyp19a2, and inhibition of vtgab, wap and cyp1a1 on exposure to 100 ng/L EE2. Lower concentrations inhibited the gene expression of vtgab and wap (50 ng/L), cyp1a1 (25 ng/L) and zp2 (12.5 ng/L). These alterations in gene expression allow us to affirm that larvae of C. variegatus are an efficient and sensitive model for biomonitoring EEDs.
AB - Currently, endocrine disruptors (EDs) can be found in all the environmental compartments. To understand the effects of estrogenic EDs (EEDs), adults of Cyprinodon variegatus have been classically used as a marine model. However, it is during development that exposure to contaminants may generate permanent consequences. Thus, the aim of this study was to verify the effects produced by acute exposure to 17α-ethinylestradiol (EE2) in C. variegatus larvae. Quantitative PCR (qPCR) results revealed the induction of vtg and zp gene expression on exposure to 1000 ng/L EE2 and the induction of vtgc, zp2, zp3 and cyp19a2, and inhibition of vtgab, wap and cyp1a1 on exposure to 100 ng/L EE2. Lower concentrations inhibited the gene expression of vtgab and wap (50 ng/L), cyp1a1 (25 ng/L) and zp2 (12.5 ng/L). These alterations in gene expression allow us to affirm that larvae of C. variegatus are an efficient and sensitive model for biomonitoring EEDs.
KW - Aromatase
KW - EE2
KW - Endocrine disruption
KW - Vitellogenin
KW - Wap
KW - Zona pellucida
UR - http://www.scopus.com/inward/record.url?scp=85144871346&partnerID=8YFLogxK
U2 - 10.1016/j.etap.2022.104042
DO - 10.1016/j.etap.2022.104042
M3 - Article
C2 - 36549414
AN - SCOPUS:85144871346
SN - 1382-6689
VL - 97
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
M1 - 104042
ER -
