TY - JOUR
T1 - A Double Payload Complex between Hypericin and All-trans Retinoic Acid in the β-Lactoglobulin Protein
AU - Rodríguez-Amigo, Beatriz
AU - Hally, Cormac
AU - Roig-Yanovsky, Núria
AU - Delcanale, Pietro
AU - Abbruzzetti, Stefania
AU - Agut, Montserrat
AU - Viappiani, Cristiano
AU - Nonell, Santi
N1 - Funding Information:
Acknowledgments: S.A. and C.V. acknowledge support from Azienda USL di Piacenza, Italy, and Fondazione di Piacenza e Vigevano. C.H. thanks the European Social Funds and the SUR del DEC de la Generalitat de Catalunya for his predoctoral fellowships (Grant No. 2017 FI_B00617, 2018 FI_B1 00174 and 2019 FI_B2 00167).
Funding Information:
Funding: This research was funded by the Spanish Ministerio de Economía y Competitividad (grant CTQ2016-78454-C2-1-R) and the Agencia Estatal de Investigación and FEDER “Una manera de hacer Europa” (PID2020-115801RB-C22/MICIN/AEI/10.13039/501100011033).
Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2022/2
Y1 - 2022/2
N2 - Combined therapies are usually used to treat acne vulgaris since this approach can tackle various foci simultaneously. Using a combination of spectroscopic, computational, and microbiological techniques and methods, herein we report on the use of β-lactoglobulin as a double payload carrier of hypericin (an antimicrobial photodynamic agent) and all-trans retinoic acid (an anti-inflammatory drug) for S. aureus in vitro photodynamic inactivation. The addition of all-trans retinoic acid to hypericinβ-lactoglobulin complex renders a photochemically safe vehicle due to the photophysical quenching of hypericin, which recovers its photodynamic activity when in contact with bacteria. The ability of hypericin to photoinactivate S. aureus was not affected by retinoic acid. β-Lactoglobulin is a novel biocompatible and photochemically safe nanovehicle with strong potential for the treatment of acne.
AB - Combined therapies are usually used to treat acne vulgaris since this approach can tackle various foci simultaneously. Using a combination of spectroscopic, computational, and microbiological techniques and methods, herein we report on the use of β-lactoglobulin as a double payload carrier of hypericin (an antimicrobial photodynamic agent) and all-trans retinoic acid (an anti-inflammatory drug) for S. aureus in vitro photodynamic inactivation. The addition of all-trans retinoic acid to hypericinβ-lactoglobulin complex renders a photochemically safe vehicle due to the photophysical quenching of hypericin, which recovers its photodynamic activity when in contact with bacteria. The ability of hypericin to photoinactivate S. aureus was not affected by retinoic acid. β-Lactoglobulin is a novel biocompatible and photochemically safe nanovehicle with strong potential for the treatment of acne.
KW - Acne vulgaris
KW - Hypericin
KW - Photodynamic therapy
KW - Retinoic acid
KW - β-lactoglobulin
UR - http://www.scopus.com/inward/record.url?scp=85125445800&partnerID=8YFLogxK
UR - http://hdl.handle.net/20.500.14342/4445
U2 - 10.3390/antibiotics11020282
DO - 10.3390/antibiotics11020282
M3 - Article
AN - SCOPUS:85125445800
SN - 2079-6382
VL - 11
JO - Antibiotics
JF - Antibiotics
IS - 2
M1 - 282
ER -
