TY - JOUR
T1 - The ArcAB two-component regulatory system promotes resistance to reactive oxygen species and systemic infection by Salmonella Typhimurium
AU - Pardo-Esté, Coral
AU - Hidalgo, Alejandro A.
AU - Aguirre, Camila
AU - Briones, Alan C.
AU - Cabezas, Carolina E.
AU - Castro-Severyn, Juan
AU - Fuentes, Juan A.
AU - Opazo, Cecilia M.
AU - Riedel, Claudia A.
AU - Otero, Carolina
AU - Pacheco, Rodrigo
AU - Valvano, Miguel A.
AU - Saavedra, Claudia P.
N1 - Funding Information:
This work was supported by "Fondo Nacional de Ciencia y Tecnologia" FONDECYT Grants #1120384 and #1160315 (to CPS), Grant PFB-16 (to RP) from "Comisión Nacional de Investigación Científica y Tecnológica de Chile" (CONICYT) and Universidad Andres Bello Nucleo Grant DI-3-17/N (to CPS), and Doctoral Fellowships were received from Comisión Nacional de Investigación Científica y Tecnológica de Chile" CONICYT (Grant 21150592 to CPE, Grant 21160858 to ACB, Grant 21151217 to JCS, and Grant 21180743 to CEC).
Funding Information:
Funding:Thisworkwassupportedby"Fondo NacionaldeCienciayTecnologia"FONDECYT Grants#1120384and#1160315(toCPS),Grant PFB-16(toRP)from"Comisio ´nNacionalde Investigacio ´ n Cientı ´ fica y Tecnolo ´ gica de Chile" (CONICYT)andUniversidadAndresBelloNucleo GrantDI-3-17/N(toCPS),andDoctoral
Publisher Copyright:
© 2018 Pardo-Esté et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/9
Y1 - 2018/9
N2 - Salmonella enterica Serovar Typhimurium (S. Typhimurium) is an intracellular bacterium that overcomes host immune system barriers for successful infection. The bacterium colonizes the proximal small intestine, penetrates the epithelial layer, and is engulfed by macrophages and neutrophils. Intracellularly, S. Typhimurium encounters highly toxic reactive oxygen species including hydrogen peroxide and hypochlorous acid. The molecular mechanisms of Salmonella resistance to intracellular oxidative stress is not completely understood. The ArcAB two-component system is a global regulatory system that responds to oxygen. In this work, we show that the ArcA response regulator participates in Salmonella adaptation to changing oxygen levels and is also involved in promoting intracellular survival in macrophages and neutrophils, enabling S. Typhimurium to successfully establish a systemic infection.
AB - Salmonella enterica Serovar Typhimurium (S. Typhimurium) is an intracellular bacterium that overcomes host immune system barriers for successful infection. The bacterium colonizes the proximal small intestine, penetrates the epithelial layer, and is engulfed by macrophages and neutrophils. Intracellularly, S. Typhimurium encounters highly toxic reactive oxygen species including hydrogen peroxide and hypochlorous acid. The molecular mechanisms of Salmonella resistance to intracellular oxidative stress is not completely understood. The ArcAB two-component system is a global regulatory system that responds to oxygen. In this work, we show that the ArcA response regulator participates in Salmonella adaptation to changing oxygen levels and is also involved in promoting intracellular survival in macrophages and neutrophils, enabling S. Typhimurium to successfully establish a systemic infection.
UR - http://www.scopus.com/inward/record.url?scp=85052899584&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0203497
DO - 10.1371/journal.pone.0203497
M3 - Article
C2 - 30180204
AN - SCOPUS:85052899584
SN - 1932-6203
VL - 13
JO - PLoS ONE
JF - PLoS ONE
IS - 9
M1 - e0203497
ER -