TY - JOUR
T1 - Bioprocess Strategies for Vitamin B12 Production by Microbial Fermentation and Its Market Applications
AU - Calvillo, Álvaro
AU - Pellicer, Teresa
AU - Carnicer, Marc
AU - Planas, Antoni
N1 - Funding Information:
This work was supported by grants PID2019-104350RB-I00 from MICINN, Spain, and an AGAUR grant 2017SGR-727 from the Generalitat de Catalunya (to A.P.). AC acknowledges a pre-doctoral contract 2018 DI 020 from AGAUR, Generalitat de Catalunya.
Publisher Copyright:
© 2022 by the authors.
PY - 2022/8
Y1 - 2022/8
N2 - Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. For this reason, finding better producer strains and optimizing their bioprocesses have been the main focus of industrial producers over the last few decades. In this review, we initially provide a historical overview of vitamin B12 research and the main biosynthetic characteristics of the two microorganism families typically used for its industrial production: several strains of Propionibacterium freudenreichii and strains related to Pseudomonas denitrificans. Later, a complete summary of the current state of vitamin B12 industrial production as well as the main advances and challenges for improving it is detailed, with a special focus on bioprocess optimization, which aims not only to increase production but also sustainability. In addition, a comprehensive list of the most important and relevant patents for the present industrial strains is provided. Finally, the potential applications of vitamin B12 in different markets are discussed.
AB - Vitamin B12 is a widely used compound in the feed and food, healthcare and medical industries that can only be produced by fermentation because of the complexity of its chemical synthesis. For this reason, finding better producer strains and optimizing their bioprocesses have been the main focus of industrial producers over the last few decades. In this review, we initially provide a historical overview of vitamin B12 research and the main biosynthetic characteristics of the two microorganism families typically used for its industrial production: several strains of Propionibacterium freudenreichii and strains related to Pseudomonas denitrificans. Later, a complete summary of the current state of vitamin B12 industrial production as well as the main advances and challenges for improving it is detailed, with a special focus on bioprocess optimization, which aims not only to increase production but also sustainability. In addition, a comprehensive list of the most important and relevant patents for the present industrial strains is provided. Finally, the potential applications of vitamin B12 in different markets are discussed.
KW - Propionibacterium freudenreichii
KW - Pseudomonas denitrificans
KW - cobalamin
KW - cyanocobalamin production
UR - http://www.scopus.com/inward/record.url?scp=85137346597&partnerID=8YFLogxK
U2 - 10.3390/bioengineering9080365
DO - 10.3390/bioengineering9080365
M3 - Review
AN - SCOPUS:85137346597
SN - 2306-5354
VL - 9
JO - Bioengineering
JF - Bioengineering
IS - 8
M1 - 365
ER -