Environmental noise has become one of the principal health risks for urban dwellers and road traffic noise (RTN) is considered to be the main source of these adverse effects. To address this problem, strategic noise maps and corresponding action plans have been developed throughout Europe in recent years in response to the European Noise Directive 2002/49/EC (END), especially in populated cities. Recently, wireless acoustic sensor networks (WASNs) have started to serve as an alternative to static noise maps to monitor urban areas by gathering environmental noise data in real time. Several studies have analysed and categorized the different acoustic environments described in the END (e.g., traffic, industrial, leisure, etc.). However, most of them have only considered the dynamic evolution of the A-weighted equivalent noise levels LAeq over different periods of time. In order to focus on the analysis of RTN solely, this paper introduces a clustering methodology to analyse and group spectro-temporal profiles of RTN collected simultaneously across an area of interest. The experiments were conducted on two acoustic databases collected during a weekday and a weekend day through WASNs deployed in the pilot areas of the LIFE+ DYNAMAP project. The results obtained show that the clustering of RTN, based on its spectro-temporal patterns, yields different solutions on weekdays and at weekends in both environments, being larger than those found in the suburban environment and lower than the number of clusters in the urban scenario.