TY - JOUR
T1 - Synthesis, photophysical characterization, and photoinduced antibacterial activity of methylene blue-loaded amino- and mannose-targeted mesoporous silica nanoparticles
AU - Planas, Oriol
AU - Bresolí-Obach, Roger
AU - Nos, Jaume
AU - Gallavardin, Thibault
AU - Ruiz-González, Rubén
AU - Agut, Montserrat
AU - Nonell, Santi
N1 - Publisher Copyright:
© 2015 by the authors; licensee MDPI.
PY - 2015/4
Y1 - 2015/4
N2 - Over the last 20 years, the number of pathogenic multi-resistant microorganisms has grown steadily, which has stimulated the search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called photodynamic inactivation, is emerging as a promising alternative to treatments based on conventional antibiotics. We have explored the effectiveness of methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in the photodynamic inactivation of two Gram negative bacteria, namely Escherichia coli and Pseudomonas aeruginosa. For E. coli, nanoparticle association clearly reduced the dark toxicity of MB while preserving its photoinactivation activity. For P. aeruginosa, a remarkable difference was observed between amino- and mannose-decorated nanoparticles. The details of singlet oxygen production in the nanoparticles have been characterized, revealing the presence of two populations of this cytotoxic species. Strong quenching of singlet oxygen within the nanoparticles is observed.
AB - Over the last 20 years, the number of pathogenic multi-resistant microorganisms has grown steadily, which has stimulated the search for new strategies to combat antimicrobial resistance. Antimicrobial photodynamic therapy (aPDT), also called photodynamic inactivation, is emerging as a promising alternative to treatments based on conventional antibiotics. We have explored the effectiveness of methylene blue-loaded targeted mesoporous silica nanoparticles (MSNP) in the photodynamic inactivation of two Gram negative bacteria, namely Escherichia coli and Pseudomonas aeruginosa. For E. coli, nanoparticle association clearly reduced the dark toxicity of MB while preserving its photoinactivation activity. For P. aeruginosa, a remarkable difference was observed between amino- and mannose-decorated nanoparticles. The details of singlet oxygen production in the nanoparticles have been characterized, revealing the presence of two populations of this cytotoxic species. Strong quenching of singlet oxygen within the nanoparticles is observed.
KW - Antimicrobial photodynamic therapy (aPDT)
KW - Drug delivery system
KW - E. Coli
KW - Mannose
KW - Mesoporous silica nanoparticle (MSNP)
KW - Methylene blue
KW - P. Aeruginosa
KW - Singlet oxygen (<sup>1</sup>O<inf>2</inf>)
UR - http://www.scopus.com/inward/record.url?scp=84928679036&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000354480700065&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.3390/molecules20046284
DO - 10.3390/molecules20046284
M3 - Article
C2 - 25859784
AN - SCOPUS:84928679036
SN - 1420-3049
VL - 20
SP - 6284
EP - 6298
JO - Molecules
JF - Molecules
IS - 4
ER -