Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus

Marc Carnicer, Gilles Vieira, Trygve Brautaset, Jean Charles Portais, Stephanie Heux

Producció científica: Article en revista indexadaArticleAvaluat per experts

19 Cites (Scopus)


Background: The gram-positive bacterium Bacillus methanolicus MGA3 is a promising candidate for methanol-based biotechnologies. Accurate determination of intracellular metabolites is crucial for engineering this bacteria into an efficient microbial cell factory. Due to the diversity of chemical and cell properties, an experimental protocol validated on B. methanolicus is needed. Here a systematic evaluation of different techniques for establishing a reliable basis for metabolome investigations is presented. Results: Metabolome analysis was focused on metabolites closely linked with B. methanolicus central methanol metabolism. As an alternative to cold solvent based procedures, a solvent-free quenching strategy using stainless steel beads cooled to 20 °C was assessed. The precision, the consistency of the measurements, and the extent of metabolite leakage from quenched cells were evaluated in procedures with and without cell separation. The most accurate and reliable performance was provided by the method without cell separation, as significant metabolite leakage occurred in the procedures based on fast filtration. As a biological test case, the best protocol was used to assess the metabolome of B. methanolicus grown in chemostat on methanol at two different growth rates and its validity was demonstrated. Conclusion: The presented protocol is a first and helpful step towards developing reliable metabolomics data for thermophilic methylotroph B. methanolicus. This will definitely help for designing an efficient methylotrophic cell factory.

Idioma originalAnglès
Número d’article92
RevistaMicrobial Cell Factories
Estat de la publicacióPublicada - 1 de juny 2016
Publicat externament


Navegar pels temes de recerca de 'Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus'. Junts formen un fingerprint únic.

Com citar-ho