TY - JOUR
T1 - A metabolomic study of red and processed meat intake and acylcarnitine concentrations in human urine and blood
AU - Wedekind, Roland
AU - Kiss, Agneta
AU - Keski-Rahkonen, Pekka
AU - Viallon, Vivian
AU - Rothwell, Joseph A.
AU - Cross, Amanda J.
AU - Rostgaard-Hansen, Agnetha Linn
AU - Sandanger, Torkjel M.
AU - Jakszyn, Paula
AU - Schmidt, Julie A.
AU - Pala, Valeria
AU - Vermeulen, Roel
AU - Schulze, Matthias B.
AU - Kühn, Tilman
AU - Johnson, Theron
AU - Trichopoulou, Antonia
AU - Peppa, Eleni
AU - Vechia, Carlo La
AU - Masala, Giovanna
AU - Tumino, Rosario
AU - Sacerdote, Carlotta
AU - Wittenbecher, Clemens
AU - de Magistris, Maria Santucci
AU - Dahm, Christina C.
AU - Severi, Gianluca
AU - Mancini, Francesca Romana
AU - Weiderpass, Elisabete
AU - Gunter, Marc J.
AU - Huybrechts, Inge
AU - Scalbert, Augustin
N1 - Funding Information:
This work was supported by the International Agency for Research on Cancer. The national cohorts are supported by 3M, Mutuelle Générale de l'Education Nationale, and Institut National de la Santé et de la Recherche Medicale (France); Deutsche Krebshilfe, Deutsches Krebsforschungszentrum (DKFZ), and the Federal Ministry of Education and Research (Germany); Hellenic Health Foundation (Greece); Italian Association for Research on Cancer (AIRC), and National Research Council (Italy). RW is supported by a stipend of La Ligue Nationale contre le Cancer (France).
Publisher Copyright:
Copyright © The Author(s) on behalf of the American Society for Nutrition.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Background: Acylcarnitines (ACs) play a major role in fatty acid metabolism and are potential markers of metabolic dysfunction with higher blood concentrations reported in obese and diabetic individuals. Diet, and in particular red and processed meat intake, has been shown to influence AC concentrations but data on the effect of meat consumption on AC concentrations is limited. Objectives: To investigate the effect of red and processed meat intake on AC concentrations in plasma and urine using a randomized controlled trial with replication in an observational cohort. Methods: In the randomized crossover trial, 12 volunteers successively consumed 2 different diets containing either pork or tofu for 3 d each. A panel of 44 ACs including several oxidized ACs was analyzed by LC-MS in plasma and urine samples collected after the 3-d period. ACs that were associated with pork intake were then measured in urine (n = 474) and serum samples (n = 451) from the European Prospective Investigation into Cancer and nutrition (EPIC) study and tested for associations with habitual red and processed meat intake derived from dietary questionnaires. Results: In urine samples from the intervention study, pork intake was positively associated with concentrations of 18 short- and medium-chain ACs. Eleven of these were also positively associated with habitual red and processed meat intake in the EPIC cross-sectional study. In blood, C18:0 was positively associated with red meat intake in both the intervention study (q = 0.004, Student's t-test) and the cross-sectional study (q = 0.033, linear regression). Conclusions: AC concentrations in urine and blood were associated with red meat intake in both a highly controlled intervention study and in subjects of a cross-sectional study. Our data on the role of meat intake on this important pathway of fatty acid and energy metabolism may help understanding the role of red meat consumption in the etiology of some chronic diseases. This trial was registered at clinicaltrials.gov as NCT03354130. Am J Clin Nutr 2020;112:381-388.
AB - Background: Acylcarnitines (ACs) play a major role in fatty acid metabolism and are potential markers of metabolic dysfunction with higher blood concentrations reported in obese and diabetic individuals. Diet, and in particular red and processed meat intake, has been shown to influence AC concentrations but data on the effect of meat consumption on AC concentrations is limited. Objectives: To investigate the effect of red and processed meat intake on AC concentrations in plasma and urine using a randomized controlled trial with replication in an observational cohort. Methods: In the randomized crossover trial, 12 volunteers successively consumed 2 different diets containing either pork or tofu for 3 d each. A panel of 44 ACs including several oxidized ACs was analyzed by LC-MS in plasma and urine samples collected after the 3-d period. ACs that were associated with pork intake were then measured in urine (n = 474) and serum samples (n = 451) from the European Prospective Investigation into Cancer and nutrition (EPIC) study and tested for associations with habitual red and processed meat intake derived from dietary questionnaires. Results: In urine samples from the intervention study, pork intake was positively associated with concentrations of 18 short- and medium-chain ACs. Eleven of these were also positively associated with habitual red and processed meat intake in the EPIC cross-sectional study. In blood, C18:0 was positively associated with red meat intake in both the intervention study (q = 0.004, Student's t-test) and the cross-sectional study (q = 0.033, linear regression). Conclusions: AC concentrations in urine and blood were associated with red meat intake in both a highly controlled intervention study and in subjects of a cross-sectional study. Our data on the role of meat intake on this important pathway of fatty acid and energy metabolism may help understanding the role of red meat consumption in the etiology of some chronic diseases. This trial was registered at clinicaltrials.gov as NCT03354130. Am J Clin Nutr 2020;112:381-388.
KW - Acylcarnitines
KW - Blood
KW - Meat intake
KW - Metabolomics
KW - Red and processed meat
KW - Urine
UR - http://www.scopus.com/inward/record.url?scp=85089128187&partnerID=8YFLogxK
U2 - 10.1093/ajcn/nqaa140
DO - 10.1093/ajcn/nqaa140
M3 - Article
C2 - 32492168
AN - SCOPUS:85089128187
SN - 0002-9165
VL - 112
SP - 381
EP - 388
JO - American Journal of Clinical Nutrition
JF - American Journal of Clinical Nutrition
IS - 2
ER -
