TY - CHAP
T1 - A Dynamic, in vitro BBB model to study the effects of varying levels of shear stress
AU - Martorell López, Jordi
AU - Balcells Camps, Mercedes
AU - Molins Gutiérrez, Gemma
AU - Salazar-Martin, Antonio G.
N1 - This work was supported in part by a grant from Spain Ministerio de Economía y Competitividad (SAF-2017-84773-C2, MB) and by the Global CoCreation Lab (GM, MB).
PY - 2022/6/1
Y1 - 2022/6/1
N2 - The blood–brain barrier (BBB) consists of a tight network of blood capillaries in the brain that separate the circulatory system from the central nervous system. Its particular properties are based on the dynamic interaction between cerebral endothelial cells and other surrounding cells, especially astrocytes. We have designed and synthesized a three-dimensional scaffold that recapitulates the main hallmarks of the BBB extracellular matrix and serves as a platform to co-culture human brain microvascular endothelial cells and human cortical astrocytes. The scaffold can be exposed to flow, thereby allowing the study of flow-mediated pathways at the BBB.
AB - The blood–brain barrier (BBB) consists of a tight network of blood capillaries in the brain that separate the circulatory system from the central nervous system. Its particular properties are based on the dynamic interaction between cerebral endothelial cells and other surrounding cells, especially astrocytes. We have designed and synthesized a three-dimensional scaffold that recapitulates the main hallmarks of the BBB extracellular matrix and serves as a platform to co-culture human brain microvascular endothelial cells and human cortical astrocytes. The scaffold can be exposed to flow, thereby allowing the study of flow-mediated pathways at the BBB.
UR - https://link.springer.com/protocol/10.1007/978-1-0716-2289-6_10
UR - http://www.scopus.com/inward/record.url?scp=85132620426&partnerID=8YFLogxK
U2 - https://doi.org/10.1007/978-1-0716-2289-6_10
DO - https://doi.org/10.1007/978-1-0716-2289-6_10
M3 - Chapter
SN - 978-1-0716-2289-6
VL - 2492
SP - 175
EP - 190
BT - The Blood-Brain Barrier
PB - Springer Nature
ER -