Ultra-performance liquid chromatography/tandem mass spectrometry for the simultaneous analysis of aflatoxins B1, G1, B2, G2 and ochratoxin A in beer

Meritxell Ventura, David Guillén, Ivan Anaya, Francesc Broto-Puig, Josep Lluís Lliberia, Montserrat Agut, Lluís Comellas

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

87 Citas (Scopus)

Resumen

The simultaneous analysis of aflatoxins B1, G1, B2, G2 and ochratoxin A in beer was carried out by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS). Mycotoxins were extracted, purified and concentrated from the beer sample in one step using a solid-phase extraction (SPE) cartridge that contained a polymeric sorbent. Optimization of different parameters, such as type of SPE sorbent, type and amount of wash solvent and pH of the sample, was carried out. The mobile phase consisted of a gradient of methanol + water (0.1% HCOOH) and a reversed-phase C18 column was used for the separation. The mass spectrometer used an electrospray ionization source operated in the positive mode to detect aflatoxins and in the negative mode to detect ochratoxin. UPLC/MS/MS is a rapid and sensitive technique that allows the separation of the five toxins in only 3.2 min. The limit of detection is 1 pg.

Idioma originalInglés
Páginas (desde-hasta)3199-3204
Número de páginas6
PublicaciónRapid Communications in Mass Spectrometry
Volumen20
N.º21
DOI
EstadoPublicada - 2006

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