Three-Step Enzymatic Remodeling of Chitin into Bioactive Chitooligomers

Zuzana Mészáros, Natalia Kulik, Lucie Petrásková, Pavla Bojarová, Antoni Planas, Vladimír Křen, Kristýna Slámová, Monica Texidó Barahona

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

1 Cita (Scopus)

Resumen

Here we describe a complex enzymatic approach to the efficient transformation of abundant waste chitin, a byproduct of the food industry, into valuable chitooligomers with a degree of polymerization (DP) ranging from 6 to 11. This method involves a three-step process: initial hydrolysis of chitin using engineered variants of a novel fungal chitinase from Talaromyces flavus to generate low-DP chitooligomers, followed by an extension to the desired DP using the high-yielding Y445N variant of β-N-acetylhexosaminidase from Aspergillus oryzae, achieving yields of up to 57%. Subsequently, enzymatic deacetylation of chitooligomers with DP 6 and 7 was accomplished using peptidoglycan deacetylase from Bacillus subtilis BsPdaC. The innovative enzymatic procedure demonstrates a sustainable and feasible route for converting waste chitin into unavailable bioactive chitooligomers potentially applicable as natural pesticides in ecological and sustainable agriculture.

Idioma originalInglés
Páginas (desde-hasta)15613-15623
Número de páginas11
PublicaciónJournal of Agricultural and Food Chemistry
Volumen72
N.º28
DOI
EstadoPublicada - jul 2024

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