TY - CHAP
T1 - Singlet oxygen quantum yield determination using chemical acceptors
AU - Bresolí-Obach, Roger
AU - Torra, Joaquim
AU - Zanocco, Renzo P.
AU - Zanocco, Antonio L.
AU - Nonell, Santi
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.
PY - 2021
Y1 - 2021
N2 - Singlet oxygen (1O2) is the first electronic excited state of molecular oxygen. Due to its non-radical and non-ionic character as well as its mild reactivity, 1O2 has a pivotal role in cell signaling processes at low concentration, yet it is cytotoxic at high concentrations. Quantifying the production of 1O2, particularly in biological systems, is therefore essential for understanding and controlling its effects. 1O2 can be produced by chemical and biological reactions, yet its most common method of production is by photosensitization, whereby an initially photoexcited molecule transfers its acquired electronic energy to the dioxygen molecule. The efficiency of this process is characterized by the 1O2 production quantum yield, ΦΔ, which can be determined by directly monitoring its intrinsic weak near-infrared phosphorescence or indirectly by trapping it with a suitable acceptor, a process that can be monitored by common analytical techniques. Indirect methods are thus very popular, yet they may lead to severe errors if used incorrectly. Herein we describe the common aspects of indirect methods and propose a general step-by-step procedure for the determination of ΦΔ values. In addition, we identify the key experimental conditions that need to be controlled to obtain meaningful results.
AB - Singlet oxygen (1O2) is the first electronic excited state of molecular oxygen. Due to its non-radical and non-ionic character as well as its mild reactivity, 1O2 has a pivotal role in cell signaling processes at low concentration, yet it is cytotoxic at high concentrations. Quantifying the production of 1O2, particularly in biological systems, is therefore essential for understanding and controlling its effects. 1O2 can be produced by chemical and biological reactions, yet its most common method of production is by photosensitization, whereby an initially photoexcited molecule transfers its acquired electronic energy to the dioxygen molecule. The efficiency of this process is characterized by the 1O2 production quantum yield, ΦΔ, which can be determined by directly monitoring its intrinsic weak near-infrared phosphorescence or indirectly by trapping it with a suitable acceptor, a process that can be monitored by common analytical techniques. Indirect methods are thus very popular, yet they may lead to severe errors if used incorrectly. Herein we describe the common aspects of indirect methods and propose a general step-by-step procedure for the determination of ΦΔ values. In addition, we identify the key experimental conditions that need to be controlled to obtain meaningful results.
KW - Photosensitizers
KW - Singlet oxygen
KW - Singlet oxygen chemical acceptors
KW - Singlet oxygen dosimetry
KW - Singlet oxygen indirect detection methods
KW - Singlet oxygen quantum yield
UR - http://www.scopus.com/inward/record.url?scp=85090174561&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-0896-8_14
DO - 10.1007/978-1-0716-0896-8_14
M3 - Chapter
C2 - 32857355
AN - SCOPUS:85090174561
T3 - Methods in Molecular Biology
SP - 165
EP - 188
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -