TY - JOUR
T1 - Salmonella mutagenicity assessment of airborne particulate matter collected from urban areas of Rio Grande do Sul State, Brazil, differing in anthropogenic influences and polycyclic aromatic hydrocarbon levels
AU - Pereira, Tatiana S.
AU - Gotor, Gemma N.
AU - Beltrami, Laiana S.
AU - Nolla, Celia G.
AU - Rocha, Jocelita A.V.
AU - Broto, Francesc P.
AU - Comellas, Lluis R.
AU - Vargas, Vera M.F.
N1 - Funding Information:
The authors are grateful to Mariana V. Coronas and Thiene Pastoriza for their aid. This research was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) , which also granted a Scientific Initiation scholarship to Laiana Beltrami and an Additional Training scholarship to Jocelita A.V. Rocha. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) granted a doctoral scholarship to Tatiana S. Pereira.
PY - 2010/9
Y1 - 2010/9
N2 - Urban areas are both major sources and major targets of air pollution. The atmospheric environment receives diverse chemical substances, including genotoxic agents that may affect human health. The purpose of this study was to evaluate and compare the atmospheric quality in two urban areas in Rio Grande do Sul State (Brazil), under the influence of greater (Site 1) or lesser (Site 2) anthropogenic sources. Polycyclic aromatic hydrocarbons (PAHs) of environmental interest were quantified and the Salmonella/microsome assay was used for the measurement of mutagenicity. Organic compounds extracted from the airborne particulate matter were analyzed by high-performance liquid chromatography (HPLC) to detect PAHs of interest (known or possible carcinogens). The organic extracts were also tested for mutagenic and cytotoxic activity in the Salmonella/microsome assay with strains TA98, TA100, YG1021 and YG1024, with or without S9 activation. At Site 1, benzo[. ghi]perylene (BghiP) and indeno[1,2,3- cd]pyrene (IP) were found at higher concentrations and mutagenicity (revertants per μg extract) varied from 1.0 ± 0.25 (TA98, no S9) to 5.2 ± 0.45 (TA98, S9). At Site 2, BghiP and IP were present in larger amounts and the mutagenic responses ranged from 0.6 to 3.7 revertants per μg (both in TA98, S9). The occurrence of BghiP and IP may be related to vehicular emissions. These and the other PAHs studied, as well as the nitro compounds, may contribute to the mutagenicity found in these airborne particles.
AB - Urban areas are both major sources and major targets of air pollution. The atmospheric environment receives diverse chemical substances, including genotoxic agents that may affect human health. The purpose of this study was to evaluate and compare the atmospheric quality in two urban areas in Rio Grande do Sul State (Brazil), under the influence of greater (Site 1) or lesser (Site 2) anthropogenic sources. Polycyclic aromatic hydrocarbons (PAHs) of environmental interest were quantified and the Salmonella/microsome assay was used for the measurement of mutagenicity. Organic compounds extracted from the airborne particulate matter were analyzed by high-performance liquid chromatography (HPLC) to detect PAHs of interest (known or possible carcinogens). The organic extracts were also tested for mutagenic and cytotoxic activity in the Salmonella/microsome assay with strains TA98, TA100, YG1021 and YG1024, with or without S9 activation. At Site 1, benzo[. ghi]perylene (BghiP) and indeno[1,2,3- cd]pyrene (IP) were found at higher concentrations and mutagenicity (revertants per μg extract) varied from 1.0 ± 0.25 (TA98, no S9) to 5.2 ± 0.45 (TA98, S9). At Site 2, BghiP and IP were present in larger amounts and the mutagenic responses ranged from 0.6 to 3.7 revertants per μg (both in TA98, S9). The occurrence of BghiP and IP may be related to vehicular emissions. These and the other PAHs studied, as well as the nitro compounds, may contribute to the mutagenicity found in these airborne particles.
KW - Mutagenicity
KW - PAHs
KW - Salmonella/microsome assay
KW - Urban airborne particulate matter
UR - http://www.scopus.com/inward/record.url?scp=77956442360&partnerID=8YFLogxK
U2 - 10.1016/j.mrgentox.2010.07.003
DO - 10.1016/j.mrgentox.2010.07.003
M3 - Article
C2 - 20643224
AN - SCOPUS:77956442360
SN - 1383-5718
VL - 702
SP - 78
EP - 85
JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
IS - 1
ER -
