Prediction and Fourier Transform Infrared Spectroscopy estimation of the secondary structure of a Bacillus licheniformis endo-β-1,3-1,4-d-glucanase

Enrique Querol, Esteve Padrós, Miquel Juncosa, Antoni Planas, Jorge Lloberas

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

The secondary structure of a recombinant Bacillus licheniformis endo-β-1,3-1,4-d-glucanase (EC.3.2.1.73) has been estimated by Fourier Transform Infrared Spectroscopy and also predicted by the algorithm of Chou and Fasman. From the curve fitting of the deconvolved IR spectrum, the most probable distribution of the secondary structural classes appears to be about 40% β-sheet, 25% reverse turn, 24% non-ordered and 11% α-helix. From theoretical prediction of secondary structure the protein would present 37 % β-sheet, 31% reverse turn, 22% non-ordered and 10% α-helix.

Idioma originalInglés
Páginas (desde-hasta)612-617
Número de páginas6
PublicaciónBiochemical and Biophysical Research Communications
Volumen184
N.º2
DOI
EstadoPublicada - 30 abr 1992

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