Poly(D, L-lactide-co-glycolide) nanoparticles as delivery agents for photodynamic therapy: Enhancing singlet oxygen release and photototoxicity by surface PEG coating

Ester Boix-Garriga, Pilar Acedo, Ana Casadó, Angeles Villanueva, Juan Carlos Stockert, Magdalena Cañete, Margarita Mora, Maria Lluïsa Sagristá, Santi Nonell

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21 Citas (Scopus)

Resumen

Poly(D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) are being considered as nanodelivery systems for photodynamic therapy. The physico-chemical and biological aspects of their use remain largely unknown. Herein we report the results of a study of PLGA NPs for the delivery of the model hydrophobic photosensitizer ZnTPP to HeLa cells. ZnTPP was encapsulated in PLGA with high efficiency and the NPs showed negative zeta potentials and diameters close to 110 nm. Poly(ethylene glycol) (PEG) coating, introduced to prevent opsonization and clearance by macrophages, decreased the size and zeta potential of the NPs by roughly a factor of two and improved their stability in the presence of serum proteins. Photophysical studies revealed two and three populations of ZnTPP and singlet oxygen in uncoated and PEGylated NPs, respectively. Singlet oxygen is confined within the NPs in bare PLGA while it is more easily released into the external medium after PEG coating, which contributes to a higher photocytotoxicity towards HeLa cells in vitro. PLGA NPs are internalized by endocytosis, deliver their cargo to lysosomes and induce cell death by apoptosis upon exposure to light. In conclusion, PLGA NPs coated with PEG show high potential as delivery systems for photodynamic applications.

Idioma originalInglés
Número de artículo365104
PublicaciónNanotechnology
Volumen26
N.º36
DOI
EstadoPublicada - 11 sept 2015

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