Neuronal prosurvival role of ceramide synthase 2 by olidogendrocyte-to-neuron extracellular vesicle transfer

Álvaro Casadomé-Perales, Sara Naya, Elisa Fernández-Martínez, Bea G. Mille, Marta Guerrero-Valero, Héctor Peinado, Francesc X. Guix, Carlos G. Dotti, Ernest Palomer

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

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Resumen

Ageing is associated with notorious alterations in neurons, i.e., in gene expression, mitochondrial function, membrane degradation or intercellular communication. However, neurons live for the entire lifespan of the individual. One of the reasons why neurons remain functional in elderly people is survival mechanisms prevail over death mechanisms. While many signals are either pro-survival or pro-death, others can play both roles. Extracellular vesicles (EVs) can signal both pro-toxicity and survival. We used young and old animals, primary neuronal and oligodendrocyte cultures and neuroblastoma and oligodendrocytic lines. We analysed our samples using a combination of proteomics and artificial neural networks, biochemistry and immunofluorescence approaches. We found an age-dependent increase in ceramide synthase 2 (CerS2) in cortical EVs, expressed by oligodendrocytes. In addition, we show that CerS2 is present in neurons via the uptake of oligodendrocyte-derived EVs. Finally, we show that age-associated inflammation and metabolic stress favour CerS2 expression and that oligodendrocyte-derived EVs loaded with CerS2 lead to the expression of the antiapoptotic factor Bcl2 in inflammatory conditions. Our study shows that intercellular communication is altered in the ageing brain, which favours neuronal survival through the transfer of oligodendrocyte-derived EVs containing CerS2.

Idioma originalInglés
Número de artículo5986
Número de páginas17
PublicaciónInternational Journal of Molecular Sciences
Volumen24
N.º6
DOI
EstadoPublicada - mar 2023

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