Microcarriers for stem cell culture

Título traducido de la contribución: Microcarriers for stem cell culture

Steve Oh (Autor), Martí Lecina Veciana (Autor), Andre Choo (Autor), Shaul Reuveny (Autor), Robert Zweigerdt (Autor), Allen Chen (Autor)

Producción científica: PatenteSolicitud PCT

Resumen

We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
Título traducido de la contribuciónMicrocarriers for stem cell culture
Idioma originalInglés
Número de patenteUS 14/134,275
Fecha de prioridad17/03/08
EstadoPublicada - 17 mar 2008

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