Lipase activity and enantioselectivity of whole cells from a wild-type Aspergillius flavus strain

Carmen Solarte, Edinson Yara-Varón, Jordi Eras, Mercè Torres, Mercè Balcells, Ramon Canela-Garayoa

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

This study reports the high enantiomeric preference of whole cell lipase from Aspergillus flavus wild-type that allows the preparation of a chiral secondary alcohol. Whole cells prepared from a wild-type Aspergillus flavus strain were used as biocatalysts to prepare (R)-1-phenylethyl acetate. (R)-1-Phenylethanol was esterified into (R)-1-phenylethyl acetate with a 94.6% enantiomeric excess (ee) within 24 h at 40 C and (S)-1-phenylethanol remained in the reaction medium with a >99%ee. Besides, this biocatalyst allows the preparation of ethyl laurate and a mixture of 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate. The ethyl laurate yield was 96%, whereas the synthesis of a mixture of the acrylate regioisomers, 2-chloro-1-(chloromethyl)ethyl acrylate and 2,3-dichloro-1-propyl acrylate gave similar yields to those obtained using commercial lipases.

Idioma originalInglés
Páginas (desde-hasta)78-83
Número de páginas6
PublicaciónJournal of Molecular Catalysis B: Enzymatic
Volumen100
DOI
EstadoPublicada - feb 2014
Publicado de forma externa

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