HEK293 cell-based bioprocess development at bench scale by means of online monitoring in shake flasks (RAMOS and SFR)

Tibor Anderlei, Michael V. Keebler, Jordi Joan Cairó, Martí Lecina

Producción científica: Capítulo del libroCapítulorevisión exhaustiva

4 Citas (Scopus)

Resumen

The platforms for bioprocess development have been developed in parallel to the needs of the manufacturing industry of biopharmaceuticals, aiming to ensure the quality and safety of their products. In this sense, Quality by Design (QbD) and Process Analytical Technology (PAT) have become the pillars for quality control and quality assurance. A new combination of Shake Flask Reader (SFR) and Respiration Activity Monitoring System for online determination of OTR and CTR (RAMOS) allows online monitoring of main culture parameters needed for bioprocess development (pH, pO2, OTR, CTR, and QR) as presented below. Eventually, a case study of the application of the combination of SFR-RAMOS system is presented. The case study discloses the optimization of HEK293 cells cultures through the manipulation of their metabolic behavior.

Idioma originalInglés
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press Inc.
Páginas83-103
Número de páginas21
DOI
EstadoPublicada - 2020

Serie de la publicación

NombreMethods in Molecular Biology
Volumen2095
ISSN (versión impresa)1064-3745
ISSN (versión digital)1940-6029

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