Functionalized, swellable hydrogel layers as a platform for cell studies

Núria Marí-Buyé, Shannan O'Shaughnessy, Carles Colominas, Carlos E. Semino, Karen K. Gleason, Salvador Borrós

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

49 Citas (Scopus)

Resumen

This paper reports the design, synthesis and characterization of thin films as a platform for studying the separate influences of physical and chemical cues of a matrix on the adhesion, growth and final phenotype of cells. Independent control of the physical and chemical properties of functionalized, swellable hydrogel thin films is achieved using initiated chemical vapor deposition (iCVD). The systematic variation in crosslink density is demonstrated to control the swelling ability ofthe iCVD hydrogel films based on 2-hydroxyethyl methacrylate (HEMA). At the same time, the incorporation of controllable concentrations of the active ester pentafluorophenyl methacrylate (PFM) allows easy immobilization of aminated bioactive motifs, such as bioactive peptides. Initial cell culture results with human umbilical vein endothelial cells (HUVEC) indicate that the strategy of using PFM to immobilize a cell- adhesion peptide motif onto the hydrogel layers promotes proper HUVEC growth and enhances their phenotype.

Idioma originalInglés
Páginas (desde-hasta)1276-1286
Número de páginas11
PublicaciónAdvanced Functional Materials
Volumen19
N.º8
DOI
EstadoPublicada - 23 abr 2009

Huella

Profundice en los temas de investigación de 'Functionalized, swellable hydrogel layers as a platform for cell studies'. En conjunto forman una huella única.

Citar esto