Engineered 3D bioimplants using elastomeric scaffold, self-assembling peptide hydrogel, and adipose tissue-derived progenitor cells for cardiac regeneration

Carolina Soler-Botija, Juli R. Bagó, Aida Llucià-Valldeperas, Ana Vallés-Lluch, Cristina Castells-Sala, Cristina Martínez-Ramos, Teresa Fernández-Muiños, Juan Carlos Chachques, Manuel Monleón Pradas Pradas, Carlos E. Semino, Antoni Bayes-Genis

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

35 Citas (Scopus)

Resumen

Contractile restoration of myocardial scars remains a challenge with important clinical implications. Here, a combination of porous elastomeric membrane, peptide hydrogel, and subcutaneous adipose tissue-derived progenitor cells (subATDPCs) was designed and evaluated as a bioimplant for cardiac regeneration in a mouse model of myocardial infarction. SubATDPCs were doubly transduced with lentiviral vectors to express bioluminescent-fluorescent reporters driven by constitutively active, cardiac tissue-specific promoters. Cells were seeded into an engineered bioimplant consisting of a scaffold (polycaprolactone methacryloyloxyethyl ester) filled with a peptide hydrogel (PuraMatrix™), and transplanted to cover injured myocardium. Bioluminescence and fluorescence quantifications showed de novo and progressive increases in promoter expression in bioactive implant-treated animals. The bioactive implant was well adapted to the heart, and fully functional vessels traversed the myocardium-bioactive implant interface. Treatment translated into a detectable positive effect on cardiac function, as revealed by echocardiography. Thus, this novel implant is a promising construct for supporting myocardial regeneration.

Idioma originalInglés
Páginas (desde-hasta)291-301
Número de páginas11
PublicaciónAmerican Journal of Translational Research
Volumen6
N.º3
EstadoPublicada - 2014

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