Development of a new high-performance liquid chromatography method to analyse N-methylcarbamate insecticides by a simple post-column derivatization system and fluorescence detection

A. Sabala, J. L. Portillo, F. Broto-Puig, L. Comellas

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

16 Citas (Scopus)

Resumen

A simple chromatographic method is proposed to analyse N-methylcarbamate insecticides by post-column derivatization and fluorescence detection. The new method performs the post-column reaction without the necessity of using any delivery pumps to introduce the reagents after the analytical column in the chromatographic system. The simplification is possible since the hydrolysis (at basic pH) and derivatization (with o-phthaldialdehyde and 2-mercaptoethanol) reagents are present in the mobile phase. Analytical columns (which tolerate basic conditions) and pH of the mobile phase were studied. Kromasil-100-C18 column and berate buffer were chosen. The length and the temperature of the tubular reactor were also optimised. Results of the validation study with the proposed chromatographic method are shown. The values demonstrated good performance of the complete system, including the post-column derivatization reaction. The column showed constant efficacy for more than 200 injections.

Idioma originalInglés
Páginas (desde-hasta)103-110
Número de páginas8
PublicaciónJournal of Chromatography A
Volumen778
N.º1-2
DOI
EstadoPublicada - 22 ago 1997

Huella

Profundice en los temas de investigación de 'Development of a new high-performance liquid chromatography method to analyse N-methylcarbamate insecticides by a simple post-column derivatization system and fluorescence detection'. En conjunto forman una huella única.

Citar esto