Chemical rescue of α3-galactosyltransferase. Implications in the mechanism of retaining glycosyltransferases

Ana Monegal, Antoni Planas

Producción científica: Artículo en revista indizadaArtículorevisión exhaustiva

53 Citas (Scopus)

Resumen

The mechanism of retaining glycosyltransferases is still poorly understood and the subject of current debate. Both double displacement and front side single displacement (SNi) mechanisms have been proposed. A "chemical rescue methodology" is here applied to a retaining α3-galactosyltransferase. Azide as exogenous nucleophile rescues the activity of the inactive E317A mutant to give β-d-galactosylazide. This result fits best with a double displacement mechanism in which Glu317 is the enzyme nucleophile involved in the formation of a glycosyl-enzyme intermediate.

Idioma originalInglés
Páginas (desde-hasta)16030-16031
Número de páginas2
PublicaciónJournal of the American Chemical Society
Volumen128
N.º50
DOI
EstadoPublicada - 20 dic 2006

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