Resumen
The mechanism of retaining glycosyltransferases is still poorly understood and the subject of current debate. Both double displacement and front side single displacement (SNi) mechanisms have been proposed. A "chemical rescue methodology" is here applied to a retaining α3-galactosyltransferase. Azide as exogenous nucleophile rescues the activity of the inactive E317A mutant to give β-d-galactosylazide. This result fits best with a double displacement mechanism in which Glu317 is the enzyme nucleophile involved in the formation of a glycosyl-enzyme intermediate.
Idioma original | Inglés |
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Páginas (desde-hasta) | 16030-16031 |
Número de páginas | 2 |
Publicación | Journal of the American Chemical Society |
Volumen | 128 |
N.º | 50 |
DOI | |
Estado | Publicada - 20 dic 2006 |