TY - JOUR
T1 - Characterization of monoaminergic neurochemicals in the different brain regions of adult zebrafish
AU - Mayol-Cabré, Marta
AU - Prats, Eva
AU - Raldúa, Demetrio
AU - Gómez-Canela, Cristian
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/11/25
Y1 - 2020/11/25
N2 - Monoaminergic neurotransmitters are the main components that regulate of a lot of processes in the vertebrate brain. There is growing interest to monitor the changes produced in these neurochemicals due to the large number of exogenous agents, such as pharmaceuticals and drugs of abuse, targeting and affecting this system. Adult zebrafish (Danio rerio) shares the common neurotransmitter pathways and nervous system organization with mammals. Therefore, a method based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed for the first time to study the profile of ten monoaminergic neurochemicals in the anterior, middle and posterior brain regions of adult zebrafish. Moreover, the applied LC-MS/MS method has been studied in terms of quality such as linearity, sensitivity and intra- and inter-day precision. The analytical method based in LC-MS/MS has become a new source in neurotoxicology using adult zebrafish as research model. Significant differences on the levels of these neurotransmitters have been found between the different brain regions. Capsule: The profile of ten monoaminergic neurochemicals in the main three brain areas of adult zebrafish has been reported for the first time in this manuscript.
AB - Monoaminergic neurotransmitters are the main components that regulate of a lot of processes in the vertebrate brain. There is growing interest to monitor the changes produced in these neurochemicals due to the large number of exogenous agents, such as pharmaceuticals and drugs of abuse, targeting and affecting this system. Adult zebrafish (Danio rerio) shares the common neurotransmitter pathways and nervous system organization with mammals. Therefore, a method based on liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been developed for the first time to study the profile of ten monoaminergic neurochemicals in the anterior, middle and posterior brain regions of adult zebrafish. Moreover, the applied LC-MS/MS method has been studied in terms of quality such as linearity, sensitivity and intra- and inter-day precision. The analytical method based in LC-MS/MS has become a new source in neurotoxicology using adult zebrafish as research model. Significant differences on the levels of these neurotransmitters have been found between the different brain regions. Capsule: The profile of ten monoaminergic neurochemicals in the main three brain areas of adult zebrafish has been reported for the first time in this manuscript.
KW - Adult zebrafish
KW - Brain
KW - Liquid chromatography
KW - Neurotransmitters
KW - Tandem mass spectrometry
UR - https://www.scopus.com/pages/publications/85088657037
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000579365600083&DestLinkType=FullRecord&DestApp=WOS_CPL
UR - http://hdl.handle.net/20.500.14342/4801
U2 - 10.1016/j.scitotenv.2020.141205
DO - 10.1016/j.scitotenv.2020.141205
M3 - Article
C2 - 32758735
AN - SCOPUS:85088657037
SN - 0048-9697
VL - 745
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 141205
ER -