Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido; Aitziber L. Cortajarena; Joaquim Torra; Rubén Ruiz-González; Santi Nonell; Cristina Flors

doi:10.1039/c6cc03119f

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Alberto Rodríguez-Pulido
, Aitziber L. Cortajarena
, Joaquim Torra
, Rubén Ruiz-González
, Santi Nonell
, Cristina Flors^*

^*Autor/a de correspondencia de este trabajo

Producción científica: Artículo en revista indizada › Artículo › revisión exhaustiva

33 Citas (Scopus)

Resumen

Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

Idioma original	Inglés
Páginas (desde-hasta)	8405-8408
Número de páginas	4
Publicación	Chemical Communications
Volumen	52
N.º	54
DOI	https://doi.org/10.1039/c6cc03119f
Estado	Publicada - 11 jul 2016

Acceder al documento

10.1039/c6cc03119f

Otros archivos y enlaces

Cómo citar

@article{a6db87b880f24ff29bc9e68d788f229f,

title = "Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy",

abstract = "Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.",

keywords = "Singlet oxygen photosensitization, Fluorescent protein ilov, Electron-microscopy, Light, Photooxidation, Cytochemistry, Photophysics, Technology, Generation, Probes",

author = "Alberto Rodr{\'i}guez-Pulido and Cortajarena, \{Aitziber L.\} and Joaquim Torra and Rub{\'e}n Ruiz-Gonz{\'a}lez and Santi Nonell and Cristina Flors",

note = "Publisher Copyright: {\textcopyright} 2016 The Royal Society of Chemistry.",

year = "2016",

month = jul,

day = "11",

doi = "10.1039/c6cc03119f",

language = "English",

volume = "52",

pages = "8405--8408",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "54",

}

TY - JOUR

T1 - Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

AU - Rodríguez-Pulido, Alberto

AU - Cortajarena, Aitziber L.

AU - Torra, Joaquim

AU - Ruiz-González, Rubén

AU - Nonell, Santi

AU - Flors, Cristina

PY - 2016/7/11

Y1 - 2016/7/11

N2 - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

AB - Photosensitizing flavoproteins have great potential as tags for correlative light and electron microscopy (CLEM). We examine the photostability of miniSOG mutants and their ability to photo-oxidize diaminobenzidine, both key aspects for CLEM. Our experiments reveal a complex relation between these parameters and the production of different reactive oxygen species.

KW - Singlet oxygen photosensitization

KW - Fluorescent protein ilov

KW - Electron-microscopy

KW - Light

KW - Photooxidation

KW - Cytochemistry

KW - Photophysics

KW - Technology

KW - Generation

KW - Probes

UR - https://www.scopus.com/pages/publications/84976585960

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000378715600014&DestLinkType=FullRecord&DestApp=WOS_CPL

U2 - 10.1039/c6cc03119f

DO - 10.1039/c6cc03119f

M3 - Article

C2 - 27301706

AN - SCOPUS:84976585960

SN - 1359-7345

VL - 52

SP - 8405

EP - 8408

JO - Chemical Communications

JF - Chemical Communications

IS - 54

ER -

Assessing the potential of photosensitizing flavoproteins as tags for correlative microscopy

Resumen

Acceder al documento

Otros archivos y enlaces

Huella

Cómo citar