Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices

Antoni Delpino-Rius, Jordi Eras, Alexis Marsol-Vall, Francisca Vilaró, Mercè Balcells, Ramon Canela-Garayoa

Research output: Indexed journal article Articlepeer-review

48 Citations (Scopus)

Abstract

We have developed an analytical method that allows the simultaneous determination of epoxycarotenoids, hydroxycarotenoids and carotenes in monovarietal fresh homemade and industrially processed fruit products. Analyses were carried out using ultra performance liquid chromatography (UPLC). The extraction method was optimized using methanol as the first extraction solvent for lyophilized samples followed by a saponification step. Recoveries ranged between 75% and 104% depending on the compound. Repeatability was better than 10% for all compounds (%RSD, n= 3). The chromatographic analysis takes less than 17. min. In this short period, up to 27 carotenoids were identified in apple, peach and pear products. The developed method allowed us to differentiate juice from six varieties of apple by their carotenoid profile. Moreover, the methodology allows us to differentiate the carotenoid profiles from commercial juices and homemade fresh peach and pear juices, as well as to study the rearrangements of 5,6- to 5,8-epoxycarotenoids.

Original languageEnglish
Pages (from-to)90-99
Number of pages10
JournalJournal of Chromatography A
Volume1331
DOIs
Publication statusPublished - 28 Feb 2014
Externally publishedYes

Keywords

  • Carotenoid profile
  • Epoxycarotenoid
  • Hydroxycarotenoid
  • Monovarietal fruit juice
  • UPLC

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