Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

JL Viladot; V Moreau; A Planas; H Driguez

doi:10.1039/a701431g

Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

JL Viladot^*, V Moreau, A Planas, H Driguez

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

41 Citations (Scopus)

Abstract

The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40% overall yield.

Original language	English
Pages (from-to)	2383-2387
Number of pages	5
Journal	Journal of the Chemical Society, Perkin Transactions 1
Issue number	16
DOIs	https://doi.org/10.1039/a701431g
Publication status	Published - 21 Aug 1997

Keywords

Site-directed mutagenesis
Alpha-amylase
Licheniformis
Oligosaccharides
Substrate
Cellulase
Catalyst
Fluoride

Access to Document

10.1039/a701431g

Cite this

@article{c0dfc5941cb548f4aca3211bd42f3994,

title = "Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides",

abstract = "The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40\% overall yield.",

keywords = "Site-directed mutagenesis, Alpha-amylase, Licheniformis, Oligosaccharides, Substrate, Cellulase, Catalyst, Fluoride",

author = "JL Viladot and V Moreau and A Planas and H Driguez",

year = "1997",

month = aug,

day = "21",

doi = "10.1039/a701431g",

language = "English",

pages = "2383--2387",

journal = "Journal of the Chemical Society, Perkin Transactions 1",

issn = "1472-7781",

publisher = "Chemical Society",

number = "16",

}

TY - JOUR

T1 - Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

AU - Viladot, JL

AU - Moreau, V

AU - Planas, A

AU - Driguez, H

PY - 1997/8/21

Y1 - 1997/8/21

N2 - The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40% overall yield.

AB - The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40% overall yield.

KW - Site-directed mutagenesis

KW - Alpha-amylase

KW - Licheniformis

KW - Oligosaccharides

KW - Substrate

KW - Cellulase

KW - Catalyst

KW - Fluoride

UR - https://www.scopus.com/pages/publications/33748645183

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:A1997XR71200020&DestLinkType=FullRecord&DestApp=WOS_CPL

U2 - 10.1039/a701431g

DO - 10.1039/a701431g

M3 - Article

AN - SCOPUS:33748645183

SN - 1472-7781

SP - 2383

EP - 2387

JO - Journal of the Chemical Society, Perkin Transactions 1

JF - Journal of the Chemical Society, Perkin Transactions 1

IS - 16

ER -

Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this