Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

JL Viladot; V Moreau; A Planas; H Driguez

doi:10.1039/a701431g

Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

JL Viladot^*
, V Moreau
, A Planas
, H Driguez

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

41 Citations (Scopus)

Abstract

The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40% overall yield.

Original language	English
Pages (from-to)	2383-2387
Number of pages	5
Journal	Journal of the Chemical Society, Perkin Transactions 1
Issue number	16
DOIs	https://doi.org/10.1039/a701431g
Publication status	Published - 21 Aug 1997

Keywords

Site-directed mutagenesis
Alpha-amylase
Licheniformis
Oligosaccharides
Substrate
Cellulase
Catalyst
Fluoride

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10.1039/a701431g

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title = "Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides",

abstract = "The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40\% overall yield.",

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T1 - Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

AU - Viladot, JL

AU - Moreau, V

AU - Planas, A

AU - Driguez, H

PY - 1997/8/21

Y1 - 1997/8/21

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AB - The title enzyme from Bacillus licheniformis has been shown to catalyse the effective autocondensation of β-laminaribiosyl fluoride, and lead to alternate 1,3-1,4-β-D-glucotetraose and -glucohexaose products. The transglycosylation using the same donor and methyl β-laminaribioside as acceptor leads to the methyl 4-O-β-laminaribiosyl-β-laminaribioside in 40% overall yield.

KW - Site-directed mutagenesis

KW - Alpha-amylase

KW - Licheniformis

KW - Oligosaccharides

KW - Substrate

KW - Cellulase

KW - Catalyst

KW - Fluoride

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JO - Journal of the Chemical Society, Perkin Transactions 1

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Transglycosylation activity of Bacillus 1,3-1,4-β-D-glucan 4-glucanohydrolases. Enzymic synthesis of alternate 1,3,-1,4-β-D-glucooligosaccharides

Abstract

Keywords

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