Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection

Ana Jiménez-Banzo, Xavier Ragàs, Peter Kapusta, Santi Nonell

Research output: Indexed journal article Reviewpeer-review

143 Citations (Scopus)

Abstract

Two recent advances in optoelectronics, namely novel near-IR sensitive photomultipliers and inexpensive yet powerful diode-pumped solid-state lasers working at kHz repetition rate, enable the time-resolved detection of singlet oxygen (O2(a1Δg)) phosphorescence in photon counting mode, thereby boosting the time-resolution, sensitivity, and dynamic range of this well-established detection technique. Principles underlying this novel approach and selected examples of applications are provided in this perspective, which illustrate the advantages over the conventional analog detection mode.

Original languageEnglish
Pages (from-to)1003-1010
Number of pages8
JournalPhotochemical and Photobiological Sciences
Volume7
Issue number9
DOIs
Publication statusPublished - 2008
Event10th School of Pure and Applied Biophysics Time-Resolved Spectroscopic Methods in Biophysics - Venice, Italy
Duration: 1 Jan 2006 → …

Keywords

  • Molecular-oxygen
  • Reactive oxygen
  • Photosensitizing properties
  • Photodynamic therapy
  • Quantum yield
  • Luminescence
  • Decay
  • Porphycenes
  • 1-delta-g
  • Kinetics

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