Surface charge tunability as a powerful strategy to control electrostatic interaction for high efficiency silencing, using tailored oligopeptide-modified poly(beta-amino ester)s (PBAEs)

Pere Dosta, Nathaly Segovia, Anna Cascante, Victor Ramos, Salvador Borrós

Research output: Indexed journal article Articlepeer-review

60 Citations (Scopus)

Abstract

Here we present an extended family of pBAEs that incorporate terminal oligopeptide moieties synthesized from both positive and negative amino acids. Polymer formulations of mixtures of negative and positive oligopeptide-modified pBAEs are capable of condensing siRNA into discrete nanoparticles. We have demonstrated that efficient delivery of nucleic acids in a cell-type dependent manner can be achieved by careful control of the pBAE formulation. In addition, our approach of adding differently charged oligopeptides to the termini of poly(β-amino ester)s is of great interest for the design of tailored complexes having specific features, such as tuneable zeta potential. We anticipate that this surface charge tunability may be a powerful strategy to control unwanted electrostatic interactions, while preserving high silencing efficiency and reduced toxicity.

Original languageEnglish
Pages (from-to)82-93
Number of pages12
JournalActa Biomaterialia
Volume20
DOIs
Publication statusPublished - 1 Jul 2015

Keywords

  • Nanoparticles
  • Non viral systems
  • Poly(beta-amino ester)s
  • Polyplexes
  • SiRNA

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