Structure elucidation of uniformly (13)C labeled small molecule natural products

Mikhail Reibarkh; Thomas P. Wyche; Josep Sauri; Tim S. Bugni; Gary E. Martin; R. Thomas Williamson

doi:10.1002/mrc.4333

Structure elucidation of uniformly (13)C labeled small molecule natural products

Mikhail Reibarkh
, Thomas P. Wyche
, Josep Sauri
, Tim S. Bugni
, Gary E. Martin
, R. Thomas Williamson

Research output: Indexed journal article › Article › peer-review

9 Citations (Scopus)

Abstract

Utilization of H-2, C-13, and N-15 isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The wide-spread availability of inexpensive, uniformly C-13 enriched glucose now makes it possible to isolate uniformly C-13 labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly C-13 labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright (C) 2015 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	996-1002
Number of pages	7
Journal	Magnetic Resonance in Chemistry
Volume	53
Issue number	12
DOIs	https://doi.org/10.1002/mrc.4333
Publication status	Published - Dec 2015
Externally published	Yes

Keywords

C-13-C-13 COSY and COSYLR
Ct-hsqc
U-C-13 labeled
Enterocin
Natural products
Structure elucidation

Access to Document

10.1002/mrc.4333

Cite this

@article{1a4e9cb1c8574f70937655ae2bb301f0,

title = "Structure elucidation of uniformly (13)C labeled small molecule natural products",

abstract = "Utilization of H-2, C-13, and N-15 isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The wide-spread availability of inexpensive, uniformly C-13 enriched glucose now makes it possible to isolate uniformly C-13 labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly C-13 labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright (C) 2015 John Wiley \& Sons, Ltd.",

keywords = "C-13-C-13 COSY and COSYLR, Ct-hsqc, U-C-13 labeled, Enterocin, Natural products, Structure elucidation",

author = "Mikhail Reibarkh and Wyche, \{Thomas P.\} and Josep Sauri and Bugni, \{Tim S.\} and Martin, \{Gary E.\} and Williamson, \{R. Thomas\}",

year = "2015",

month = dec,

doi = "10.1002/mrc.4333",

language = "English",

volume = "53",

pages = "996--1002",

journal = "Magnetic Resonance in Chemistry",

issn = "0749-1581",

publisher = "John Wiley and Sons Ltd",

number = "12",

}

TY - JOUR

T1 - Structure elucidation of uniformly (13)C labeled small molecule natural products

AU - Reibarkh, Mikhail

AU - Wyche, Thomas P.

AU - Sauri, Josep

AU - Bugni, Tim S.

AU - Martin, Gary E.

AU - Williamson, R. Thomas

PY - 2015/12

Y1 - 2015/12

N2 - Utilization of H-2, C-13, and N-15 isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The wide-spread availability of inexpensive, uniformly C-13 enriched glucose now makes it possible to isolate uniformly C-13 labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly C-13 labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright (C) 2015 John Wiley & Sons, Ltd.

AB - Utilization of H-2, C-13, and N-15 isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The wide-spread availability of inexpensive, uniformly C-13 enriched glucose now makes it possible to isolate uniformly C-13 labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly C-13 labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright (C) 2015 John Wiley & Sons, Ltd.

KW - C-13-C-13 COSY and COSYLR

KW - Ct-hsqc

KW - U-C-13 labeled

KW - Enterocin

KW - Natural products

KW - Structure elucidation

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000365383400001&DestLinkType=FullRecord&DestApp=WOS_CPL

U2 - 10.1002/mrc.4333

DO - 10.1002/mrc.4333

M3 - Article

C2 - 26768304

SN - 0749-1581

VL - 53

SP - 996

EP - 1002

JO - Magnetic Resonance in Chemistry

JF - Magnetic Resonance in Chemistry

IS - 12

ER -

Structure elucidation of uniformly (13)C labeled small molecule natural products

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this