Structure elucidation of uniformly (13)C labeled small molecule natural products

Mikhail Reibarkh; Thomas P. Wyche; Josep Sauri; Tim S. Bugni; Gary E. Martin; R. Thomas Williamson

doi:10.1002/mrc.4333

Structure elucidation of uniformly (13)C labeled small molecule natural products

Mikhail Reibarkh, Thomas P. Wyche, Josep Sauri, Tim S. Bugni, Gary E. Martin, R. Thomas Williamson

Research output: Indexed journal article › Article › peer-review

6 Citations (Scopus)

Abstract

Utilization of H-2, C-13, and N-15 isotopically labeled proteins and peptides is now routine in biomolecular NMR investigations. The wide-spread availability of inexpensive, uniformly C-13 enriched glucose now makes it possible to isolate uniformly C-13 labeled natural products from microbial fermentation. We now wish to describe an approach for the rapid structural characterization of uniformly C-13 labeled natural products that avoids the pitfalls of relying on parameters typically employed in biomolecular NMR studies. Copyright (C) 2015 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	996-1002
Number of pages	7
Journal	Magnetic Resonance in Chemistry
Volume	53
Issue number	12
DOIs	https://doi.org/10.1002/mrc.4333
Publication status	Published - Dec 2015
Externally published	Yes

Keywords

C-13-C-13 COSY and COSYLR
Ct-hsqc
U-C-13 labeled
Enterocin
Natural products
Structure elucidation

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10.1002/mrc.4333

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AU - Martin, Gary E.

AU - Williamson, R. Thomas

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Structure elucidation of uniformly (13)C labeled small molecule natural products

Abstract

Keywords

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