Reactive polymer coatings: A first step toward surface engineering of microfluidic devices

Jörg Lahann, Mercedes Balcells, Hang Lu, Teresa Rodon, Klavs F. Jensen, Robert Langer

Research output: Indexed journal article Articlepeer-review

171 Citations (Scopus)

Abstract

We report fabrication, characterization, and use of micro-fluidic analysis devices containing surface-immobilized cell-capturing molecules. Amino-terminated biotin ligands are immobilized onto the luminal surface of a microdevice and effectively support self-assembly of proteins, antibodies, and mammalian cells. For this purpose, chemical vapor deposition (CVD) polymerization is used to functionalize PDMS-made microfluidic devices with poly-[para-xylylene carboxylic acid pentafluorophenolester-co-para-xylylene]. The resulting reactive coating shows excellent adhesion when deposited in thin films (∼100 nm, and the distribution of the pentafluorophenol ester groups is reasonably uniform within the microchannel inner surface, as examined by fluorescence microscopy. The utility of these devices for cell-based bioassays is demonstrated by monitoring the concentration-dependent effect of the disintegrin echistatin on cell adhesion. The described assay format could be relevant to clinical research in various fields, including angiogenesis research.

Original languageEnglish
Pages (from-to)2117-2122
Number of pages6
JournalAnalytical Chemistry
Volume75
Issue number9
DOIs
Publication statusPublished - 1 May 2003

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