Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies

Mercedes Balcells; Doris Klee; Marlies Fabry; Hartwig Höcker

doi:10.1006/jcis.1999.6527

Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies

Mercedes Balcells
, Doris Klee
, Marlies Fabry
, Hartwig Höcker^*

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

18 Citations (Scopus)

Abstract

Protein adsorption at polymer surfaces has been investigated by means of both ELISA and radiolabeling techniques. Most of the data obtained are linearly related to each other for protein concentrations between 0.01 and 1 μg/ml, i.e., the concentration range in which the maximum amount of adsorbed active protein (ELISA) is achieved. The correlation of ELISA data with radioisotope-based measurements allows quantification of the former. Specific correlation factors are described. Adsorption is shown to be strongly dependent on the polymer/protein system.

Original language	English
Pages (from-to)	198-204
Number of pages	7
Journal	Journal of Colloid and Interface Science
Volume	220
Issue number	2
DOIs	https://doi.org/10.1006/jcis.1999.6527
Publication status	Published - 15 Dec 1999
Externally published	Yes

Keywords

Immunoassay
Protein adsorption
Quantification

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title = "Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies",

abstract = "Protein adsorption at polymer surfaces has been investigated by means of both ELISA and radiolabeling techniques. Most of the data obtained are linearly related to each other for protein concentrations between 0.01 and 1 μg/ml, i.e., the concentration range in which the maximum amount of adsorbed active protein (ELISA) is achieved. The correlation of ELISA data with radioisotope-based measurements allows quantification of the former. Specific correlation factors are described. Adsorption is shown to be strongly dependent on the polymer/protein system.",

keywords = "Immunoassay, Protein adsorption, Quantification",

author = "Mercedes Balcells and Doris Klee and Marlies Fabry and Hartwig H{\"o}cker",

note = "Funding Information: This work was supported by a grant from DAAD and by the German Federal Ministerial for Education, Science, Research and Technology (BMBF), No 3N04903.",

year = "1999",

month = dec,

day = "15",

doi = "10.1006/jcis.1999.6527",

language = "English",

volume = "220",

pages = "198--204",

journal = "Journal of Colloid and Interface Science",

issn = "0021-9797",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies

AU - Balcells, Mercedes

AU - Klee, Doris

AU - Fabry, Marlies

AU - Höcker, Hartwig

N1 - Funding Information: This work was supported by a grant from DAAD and by the German Federal Ministerial for Education, Science, Research and Technology (BMBF), No 3N04903.

PY - 1999/12/15

Y1 - 1999/12/15

N2 - Protein adsorption at polymer surfaces has been investigated by means of both ELISA and radiolabeling techniques. Most of the data obtained are linearly related to each other for protein concentrations between 0.01 and 1 μg/ml, i.e., the concentration range in which the maximum amount of adsorbed active protein (ELISA) is achieved. The correlation of ELISA data with radioisotope-based measurements allows quantification of the former. Specific correlation factors are described. Adsorption is shown to be strongly dependent on the polymer/protein system.

AB - Protein adsorption at polymer surfaces has been investigated by means of both ELISA and radiolabeling techniques. Most of the data obtained are linearly related to each other for protein concentrations between 0.01 and 1 μg/ml, i.e., the concentration range in which the maximum amount of adsorbed active protein (ELISA) is achieved. The correlation of ELISA data with radioisotope-based measurements allows quantification of the former. Specific correlation factors are described. Adsorption is shown to be strongly dependent on the polymer/protein system.

KW - Immunoassay

KW - Protein adsorption

KW - Quantification

UR - https://www.scopus.com/pages/publications/0033572504

U2 - 10.1006/jcis.1999.6527

DO - 10.1006/jcis.1999.6527

M3 - Article

AN - SCOPUS:0033572504

SN - 0021-9797

VL - 220

SP - 198

EP - 204

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

IS - 2

ER -

Quantitative assessment of protein adsorption by combination of the enzyme-linked immunosorbent assay with radioisotope-based studies

Abstract

Keywords

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