Poly(D, L-lactide-co-glycolide) nanoparticles as delivery agents for photodynamic therapy: Enhancing singlet oxygen release and photototoxicity by surface PEG coating

Ester Boix-Garriga, Pilar Acedo, Ana Casadó, Angeles Villanueva, Juan Carlos Stockert, Magdalena Cañete, Margarita Mora, Maria Lluïsa Sagristá, Santi Nonell

Research output: Indexed journal article Articlepeer-review

21 Citations (Scopus)

Abstract

Poly(D, L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) are being considered as nanodelivery systems for photodynamic therapy. The physico-chemical and biological aspects of their use remain largely unknown. Herein we report the results of a study of PLGA NPs for the delivery of the model hydrophobic photosensitizer ZnTPP to HeLa cells. ZnTPP was encapsulated in PLGA with high efficiency and the NPs showed negative zeta potentials and diameters close to 110 nm. Poly(ethylene glycol) (PEG) coating, introduced to prevent opsonization and clearance by macrophages, decreased the size and zeta potential of the NPs by roughly a factor of two and improved their stability in the presence of serum proteins. Photophysical studies revealed two and three populations of ZnTPP and singlet oxygen in uncoated and PEGylated NPs, respectively. Singlet oxygen is confined within the NPs in bare PLGA while it is more easily released into the external medium after PEG coating, which contributes to a higher photocytotoxicity towards HeLa cells in vitro. PLGA NPs are internalized by endocytosis, deliver their cargo to lysosomes and induce cell death by apoptosis upon exposure to light. In conclusion, PLGA NPs coated with PEG show high potential as delivery systems for photodynamic applications.

Original languageEnglish
Article number365104
JournalNanotechnology
Volume26
Issue number36
DOIs
Publication statusPublished - 11 Sept 2015

Keywords

  • PLGA
  • nanoparticle
  • photodynamic therapy
  • photosensitizer
  • polyethyleneglycol
  • singlet oxygen

Fingerprint

Dive into the research topics of 'Poly(D, L-lactide-co-glycolide) nanoparticles as delivery agents for photodynamic therapy: Enhancing singlet oxygen release and photototoxicity by surface PEG coating'. Together they form a unique fingerprint.

Cite this