Physicochemical interaction of a lipophilic derivative of HAV antigen VP3(110-121) with lipid monolayers

P. Sospedra; I. Haro; M. A. Alsina; F. Reig; C. Mestres

doi:10.1016/S0928-4931(99)00055-7

Physicochemical interaction of a lipophilic derivative of HAV antigen VP3(110-121) with lipid monolayers

P. Sospedra, I. Haro, M. A. Alsina, F. Reig, C. Mestres

Research output: Indexed journal article › Article › peer-review

7 Citations (Scopus)

Abstract

The synthesis of a palmitoyl derivative of a peptide corresponding to the HAV-VP3(110-121) sequence is described. Physicochemical characterisation was carried out through its surface activity, penetration in monolayers and influence in the miscibility of DPPC/DPPG and DPPC/SA monolayers. This peptide showed a lower surface activity than its parent, non acylated sequence. This fact is attributed to its tendency to form aggregates in water media. The thermodynamic parameters associated to the miscibility of lipids with or without peptide have been calculated, indicating that the energies involved are very low. This implies that the peptide sequence can be inserted to monolayers by means of extension bilayers without altering the packing of these lipids. It was not possible to know if these mixed monolayers were completely miscible or not due to the lack of collapse points in the compression isotherms.

Original language	English
Pages (from-to)	543-549
Number of pages	7
Journal	Materials Science and Engineering C
Volume	8-9
DOIs	https://doi.org/10.1016/S0928-4931(99)00055-7
Publication status	Published - 1 Dec 1999
Externally published	Yes

Keywords

HAV
Monolayers
Synthetic vaccines

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0928-4931(99)00055-7

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title = "Physicochemical interaction of a lipophilic derivative of HAV antigen VP3(110-121) with lipid monolayers",

abstract = "The synthesis of a palmitoyl derivative of a peptide corresponding to the HAV-VP3(110-121) sequence is described. Physicochemical characterisation was carried out through its surface activity, penetration in monolayers and influence in the miscibility of DPPC/DPPG and DPPC/SA monolayers. This peptide showed a lower surface activity than its parent, non acylated sequence. This fact is attributed to its tendency to form aggregates in water media. The thermodynamic parameters associated to the miscibility of lipids with or without peptide have been calculated, indicating that the energies involved are very low. This implies that the peptide sequence can be inserted to monolayers by means of extension bilayers without altering the packing of these lipids. It was not possible to know if these mixed monolayers were completely miscible or not due to the lack of collapse points in the compression isotherms.",

keywords = "HAV, Monolayers, Synthetic vaccines",

author = "P. Sospedra and I. Haro and Alsina, {M. A.} and F. Reig and C. Mestres",

note = "Funding Information: This work was supported by grants BIO 95-0061-C03-02 and BIO 95-0061-C03-03 from CICYT, Spain and a predoctoral CIRIT grant (1997 FI000073) awarded to P. Sospedra.",

year = "1999",

month = dec,

day = "1",

doi = "10.1016/S0928-4931(99)00055-7",

language = "English",

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pages = "543--549",

journal = "Materials Science and Engineering C",

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TY - JOUR

T1 - Physicochemical interaction of a lipophilic derivative of HAV antigen VP3(110-121) with lipid monolayers

AU - Sospedra, P.

AU - Haro, I.

AU - Alsina, M. A.

AU - Reig, F.

AU - Mestres, C.

N1 - Funding Information: This work was supported by grants BIO 95-0061-C03-02 and BIO 95-0061-C03-03 from CICYT, Spain and a predoctoral CIRIT grant (1997 FI000073) awarded to P. Sospedra.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - The synthesis of a palmitoyl derivative of a peptide corresponding to the HAV-VP3(110-121) sequence is described. Physicochemical characterisation was carried out through its surface activity, penetration in monolayers and influence in the miscibility of DPPC/DPPG and DPPC/SA monolayers. This peptide showed a lower surface activity than its parent, non acylated sequence. This fact is attributed to its tendency to form aggregates in water media. The thermodynamic parameters associated to the miscibility of lipids with or without peptide have been calculated, indicating that the energies involved are very low. This implies that the peptide sequence can be inserted to monolayers by means of extension bilayers without altering the packing of these lipids. It was not possible to know if these mixed monolayers were completely miscible or not due to the lack of collapse points in the compression isotherms.

AB - The synthesis of a palmitoyl derivative of a peptide corresponding to the HAV-VP3(110-121) sequence is described. Physicochemical characterisation was carried out through its surface activity, penetration in monolayers and influence in the miscibility of DPPC/DPPG and DPPC/SA monolayers. This peptide showed a lower surface activity than its parent, non acylated sequence. This fact is attributed to its tendency to form aggregates in water media. The thermodynamic parameters associated to the miscibility of lipids with or without peptide have been calculated, indicating that the energies involved are very low. This implies that the peptide sequence can be inserted to monolayers by means of extension bilayers without altering the packing of these lipids. It was not possible to know if these mixed monolayers were completely miscible or not due to the lack of collapse points in the compression isotherms.

KW - HAV

KW - Monolayers

KW - Synthetic vaccines

UR - http://www.scopus.com/inward/record.url?scp=0005489659&partnerID=8YFLogxK

U2 - 10.1016/S0928-4931(99)00055-7

DO - 10.1016/S0928-4931(99)00055-7

M3 - Article

AN - SCOPUS:0005489659

SN - 0928-4931

VL - 8-9

SP - 543

EP - 549

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

ER -

Physicochemical interaction of a lipophilic derivative of HAV antigen VP3(110-121) with lipid monolayers

Abstract

Keywords

UN SDGs

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