TY - JOUR
T1 - Optimization of the method of detection of metabolites produced by the Alternaria genus
T2 - Alternariol, alternariol monomethyl ether, altenuene, altertoxin I and tentoxin
AU - Fàbrega, A.
AU - Agut, M.
AU - Calvo, M. A.
PY - 2002
Y1 - 2002
N2 - A simple method has been developed in this work for the detection of alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), altertoxin I (ATX-I), tenuazonic acid (TA), and tentoxin by means of thin-layer chromatography from cultures of several strains of Alternaria. Strains were incubated in 2% malt extract broth for 7 d at 25°C. For extraction of metabolites, chloroform was used. The solvent system benzene/methanol/acetic acid (92:6:2) was chosen. TLC plates were observed under ultraviolet light (254/366 nm). Results show that A. alternata IMI 354942 produced AOH, AME, ALT, ATX-I, tentoxin, and TA; A. alternata IMI 354943, AOH, AME, and TA; A. triticina IMI 289962 tentoxin; and A. triticina IMI 289680 AME, ATX-I, and TA.
AB - A simple method has been developed in this work for the detection of alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), altertoxin I (ATX-I), tenuazonic acid (TA), and tentoxin by means of thin-layer chromatography from cultures of several strains of Alternaria. Strains were incubated in 2% malt extract broth for 7 d at 25°C. For extraction of metabolites, chloroform was used. The solvent system benzene/methanol/acetic acid (92:6:2) was chosen. TLC plates were observed under ultraviolet light (254/366 nm). Results show that A. alternata IMI 354942 produced AOH, AME, ALT, ATX-I, tentoxin, and TA; A. alternata IMI 354943, AOH, AME, and TA; A. triticina IMI 289962 tentoxin; and A. triticina IMI 289680 AME, ATX-I, and TA.
KW - Altenuene
KW - Alternariol
KW - Alternariol monomethyl ether
KW - Altertoxin I
KW - Tentoxin
UR - http://www.scopus.com/inward/record.url?scp=0036215857&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2621.2002.tb10680.x
DO - 10.1111/j.1365-2621.2002.tb10680.x
M3 - Article
AN - SCOPUS:0036215857
SN - 0022-1147
VL - 67
SP - 802
EP - 806
JO - Journal of Food Science
JF - Journal of Food Science
IS - 2
ER -