On-line monitoring of yeast cell growth by impedance spectroscopy

A. Soley, M. Lecina, X. Gámez, J. J. Cairó, P. Riu, X. Rosell, R. Bragós, F. Gòdia

Research output: Indexed journal article Articlepeer-review

68 Citations (Scopus)


The application of impedance spectroscopy to estimate on-line cell concentration was studied. The estimation was based on the relative variation between electrical impedance measured at low (10 kHz) and high frequencies (10 MHz). Studies were carried out to characterise the influence of changes in physical and chemical parameters on the impedance measurement. Two different possibilities to perform on-line measurements were tested: a simple set-up, based on an in situ probe, gave good results but was not suitable for high agitation and aeration rates. An ex situ flow-through on-line measuring cell was used to overcome these problems, showing a better performance. The use of this set-up for the growth monitorisation of a Saccharomyces cerevisiae culture showed an efficient performance, having the correlation between estimated and measured S. cerevisiae a Pearson coefficient of 0.999.

Original languageEnglish
Pages (from-to)398-405
Number of pages8
JournalJournal of Biotechnology
Issue number4
Publication statusPublished - 10 Sept 2005
Externally publishedYes


  • Biomass estimator
  • Impedance spectroscopy
  • On-line monitoring


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