Multiple automated minibioreactor system for multifunctional screening in biotechnology

A. Fontova, A. Soley, J. Gálvez, E. Sarró, M. Lecina, J. Rosell, P. Riu, J. Cairó, F. Gòdia, R. Bragós

Research output: Book chapterConference contributionpeer-review

5 Citations (Scopus)

Abstract

The current techniques applied in biotechnology allow to obtain many types of molecules that must be tested on cell cultures (High Throughput Screening HTS). Although such tests are usually carried out automatically on mini or microwell plates, the procedures in the preindustrial stage are performed almost manually on higher volume recipients known as bioreactors. The growth conditions in both stages are completely different. The screening system presented in this work is based on the multiwell test plates philosophy, a disposable multiple minibioreactor that allows reproduction of industrial bioreactor culture conditions: aeration, stirring, temperature, O2, pH and visible range optical absorbance measurements. It is possible to reproduce the growth conditions for both suspended and adherent animal cell types using 1 to 10 ml vol. bioreactors. In the case of bacteria or yeast, it is not possible to achieve a high biomass concentration, due to the reduced head volume air supply.

Original languageEnglish
Title of host publication28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Pages632-635
Number of pages4
DOIs
Publication statusPublished - 2006
Externally publishedYes
Event28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States
Duration: 30 Aug 20063 Sept 2006

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)0589-1019

Conference

Conference28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Country/TerritoryUnited States
CityNew York, NY
Period30/08/063/09/06

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