TY - GEN
T1 - Multiple automated minibioreactor system for multifunctional screening in biotechnology
AU - Fontova, A.
AU - Soley, A.
AU - Gálvez, J.
AU - Sarró, E.
AU - Lecina, M.
AU - Rosell, J.
AU - Riu, P.
AU - Cairó, J.
AU - Gòdia, F.
AU - Bragós, R.
PY - 2006
Y1 - 2006
N2 - The current techniques applied in biotechnology allow to obtain many types of molecules that must be tested on cell cultures (High Throughput Screening HTS). Although such tests are usually carried out automatically on mini or microwell plates, the procedures in the preindustrial stage are performed almost manually on higher volume recipients known as bioreactors. The growth conditions in both stages are completely different. The screening system presented in this work is based on the multiwell test plates philosophy, a disposable multiple minibioreactor that allows reproduction of industrial bioreactor culture conditions: aeration, stirring, temperature, O2, pH and visible range optical absorbance measurements. It is possible to reproduce the growth conditions for both suspended and adherent animal cell types using 1 to 10 ml vol. bioreactors. In the case of bacteria or yeast, it is not possible to achieve a high biomass concentration, due to the reduced head volume air supply.
AB - The current techniques applied in biotechnology allow to obtain many types of molecules that must be tested on cell cultures (High Throughput Screening HTS). Although such tests are usually carried out automatically on mini or microwell plates, the procedures in the preindustrial stage are performed almost manually on higher volume recipients known as bioreactors. The growth conditions in both stages are completely different. The screening system presented in this work is based on the multiwell test plates philosophy, a disposable multiple minibioreactor that allows reproduction of industrial bioreactor culture conditions: aeration, stirring, temperature, O2, pH and visible range optical absorbance measurements. It is possible to reproduce the growth conditions for both suspended and adherent animal cell types using 1 to 10 ml vol. bioreactors. In the case of bacteria or yeast, it is not possible to achieve a high biomass concentration, due to the reduced head volume air supply.
UR - http://www.scopus.com/inward/record.url?scp=34047100945&partnerID=8YFLogxK
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000247284706192&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1109/IEMBS.2006.260628
DO - 10.1109/IEMBS.2006.260628
M3 - Conference contribution
C2 - 17946845
AN - SCOPUS:34047100945
SN - 1424400325
SN - 9781424400324
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 632
EP - 635
BT - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
T2 - 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Y2 - 30 August 2006 through 3 September 2006
ER -