Miscibility of an acylated hepatitis A synthetic antigen derivative [palmitoyl VP3(110-121)] with lipids: A monolayer study

P. Sospedra, M. Espina, M. Castro, S. Corrales, I. Haro, C. Mestres

Research output: Indexed journal article Articlepeer-review

2 Citations (Scopus)

Abstract

Mixed monolayers of an acylated derivative of hepatitis A synthetic peptide VP3(110-121) with neutral, cationic or anionic lipids were spread at the air/water interface. Deviations from ideality as well as thermodynamic values were calculated at different surface pressures using the free-excess energy, the interaction parameter and the enthalpy. The miscibility at the collapse point was also checked. Maximum deviations from ideality were found for mixtures containing the anionic lipid phosphatidylglycerol (PG), and it seems that the monolayer composition is not stable through compression, as the peptide is ejected from the film. Films containing neutral [dipalmitoylphosphatidylcholine (DPPC)] or cationic [stearylamine (SA)] lipids showed more regular behaviour. As the peptide has a net negative charge it is probable that electrostatic interactions are in part responsible of the good miscibility of palmitoyl VP3(110-121) with SA. In order to prepare liposomes containing palmitoyl VP3(110-121), lipids such as SA or DPPC/SA will be a more suitable choice than anionic lipids such as PG.

Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalColloid and Polymer Science
Volume279
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Keywords

  • Hepatitis A
  • Membrane lipids
  • Monolayers
  • Vaccines

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