Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments

Claudia Rivetti; Cristian Gómez-Canela; Silvia Lacorte; Carlos Barata

doi:10.1007/s00216-014-8308-4

Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments

Claudia Rivetti
, Cristian Gómez-Canela
, Silvia Lacorte
, Carlos Barata^*

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

11 Citations (Scopus)

Abstract

Microcystins, anatoxins and okadaic acid are toxins produced by freshwater cyanobacteria and marine dinoflagellates. These toxins have been the responsible for the illness and death of biota and humans. To determine their presence in water during blooms, sensitive analytical methods are needed. In this study, we have developed a new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended material and sediment samples. For each target compound, two selected reaction monitoring (SRM) transitions were optimised. Chromatographic conditions were optimised considering that the compounds analysed had different chemical structure and chromatographic behaviour. Using a Luna C18 column and specific SRM transitions, five phytotoxins were resolved. Method detection limits (MDL) for anatoxin-a, microcystins RR, LR and YR and okadaic acid were 7.1, 3.3, 81.7, 102.8 and 28.8 ng g^-1 dry weight in sediment, respectively. The developed analytical method was successfully applied to analyse the presence of toxins in suspended solids and sediment from Ebro River (NE Spain) and Ebro delta-associated lagoons. Anatoxin-a was detected downstream of the Riba-Roja reservoir with levels ranging from 20 to 1120 ng g^-1 dry weight of suspended solids. Okadaic acid was only detected in three samples collected in the Alfacs Bay (Ebro delta, Spain) affected by Dinophysis blooms in 2012.

Original language	English
Pages (from-to)	1451-1462
Number of pages	12
Journal	Analytical and Bioanalytical Chemistry
Volume	407
Issue number	5
DOIs	https://doi.org/10.1007/s00216-014-8308-4
Publication status	Published - 6 Feb 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Keywords

Cyanotoxins
Ebro
LC-MS/MS
Okadaic acid
Sediment

Access to Document

10.1007/s00216-014-8308-4

Cite this

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title = "Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments",

abstract = "Microcystins, anatoxins and okadaic acid are toxins produced by freshwater cyanobacteria and marine dinoflagellates. These toxins have been the responsible for the illness and death of biota and humans. To determine their presence in water during blooms, sensitive analytical methods are needed. In this study, we have developed a new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended material and sediment samples. For each target compound, two selected reaction monitoring (SRM) transitions were optimised. Chromatographic conditions were optimised considering that the compounds analysed had different chemical structure and chromatographic behaviour. Using a Luna C18 column and specific SRM transitions, five phytotoxins were resolved. Method detection limits (MDL) for anatoxin-a, microcystins RR, LR and YR and okadaic acid were 7.1, 3.3, 81.7, 102.8 and 28.8 ng g-1 dry weight in sediment, respectively. The developed analytical method was successfully applied to analyse the presence of toxins in suspended solids and sediment from Ebro River (NE Spain) and Ebro delta-associated lagoons. Anatoxin-a was detected downstream of the Riba-Roja reservoir with levels ranging from 20 to 1120 ng g-1 dry weight of suspended solids. Okadaic acid was only detected in three samples collected in the Alfacs Bay (Ebro delta, Spain) affected by Dinophysis blooms in 2012.",

keywords = "Cyanotoxins, Ebro, LC-MS/MS, Okadaic acid, Sediment",

author = "Claudia Rivetti and Cristian G{\'o}mez-Canela and Silvia Lacorte and Carlos Barata",

note = "Funding Information: This work was supported by the Spanish MICINN grant and FEDER funds (CTM2011-30471-C02-01). Claudia Rivetti was supported by a fellowship from the MICINN (FPI BES-2012-053631). We thank Jorge Diog{\`e}ne, head of the Marine Monitoring Subprogram of IRTA, Sant Carles de la R{\`a}pita, 43540, Spain for kindly supplying the Dinophysis bloom samples. Publisher Copyright: {\textcopyright} 2014 Springer-Verlag Berlin Heidelberg.",

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doi = "10.1007/s00216-014-8308-4",

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Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments. / Rivetti, Claudia; Gómez-Canela, Cristian; Lacorte, Silvia et al.
In: Analytical and Bioanalytical Chemistry, Vol. 407, No. 5, 06.02.2015, p. 1451-1462.

Research output: Indexed journal article › Article › peer-review

TY - JOUR

T1 - Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments

AU - Rivetti, Claudia

AU - Gómez-Canela, Cristian

AU - Lacorte, Silvia

AU - Barata, Carlos

N1 - Funding Information: This work was supported by the Spanish MICINN grant and FEDER funds (CTM2011-30471-C02-01). Claudia Rivetti was supported by a fellowship from the MICINN (FPI BES-2012-053631). We thank Jorge Diogène, head of the Marine Monitoring Subprogram of IRTA, Sant Carles de la Ràpita, 43540, Spain for kindly supplying the Dinophysis bloom samples. Publisher Copyright: © 2014 Springer-Verlag Berlin Heidelberg.

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N2 - Microcystins, anatoxins and okadaic acid are toxins produced by freshwater cyanobacteria and marine dinoflagellates. These toxins have been the responsible for the illness and death of biota and humans. To determine their presence in water during blooms, sensitive analytical methods are needed. In this study, we have developed a new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended material and sediment samples. For each target compound, two selected reaction monitoring (SRM) transitions were optimised. Chromatographic conditions were optimised considering that the compounds analysed had different chemical structure and chromatographic behaviour. Using a Luna C18 column and specific SRM transitions, five phytotoxins were resolved. Method detection limits (MDL) for anatoxin-a, microcystins RR, LR and YR and okadaic acid were 7.1, 3.3, 81.7, 102.8 and 28.8 ng g-1 dry weight in sediment, respectively. The developed analytical method was successfully applied to analyse the presence of toxins in suspended solids and sediment from Ebro River (NE Spain) and Ebro delta-associated lagoons. Anatoxin-a was detected downstream of the Riba-Roja reservoir with levels ranging from 20 to 1120 ng g-1 dry weight of suspended solids. Okadaic acid was only detected in three samples collected in the Alfacs Bay (Ebro delta, Spain) affected by Dinophysis blooms in 2012.

AB - Microcystins, anatoxins and okadaic acid are toxins produced by freshwater cyanobacteria and marine dinoflagellates. These toxins have been the responsible for the illness and death of biota and humans. To determine their presence in water during blooms, sensitive analytical methods are needed. In this study, we have developed a new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for fast multiresidue determination of five toxins in suspended material and sediment samples. For each target compound, two selected reaction monitoring (SRM) transitions were optimised. Chromatographic conditions were optimised considering that the compounds analysed had different chemical structure and chromatographic behaviour. Using a Luna C18 column and specific SRM transitions, five phytotoxins were resolved. Method detection limits (MDL) for anatoxin-a, microcystins RR, LR and YR and okadaic acid were 7.1, 3.3, 81.7, 102.8 and 28.8 ng g-1 dry weight in sediment, respectively. The developed analytical method was successfully applied to analyse the presence of toxins in suspended solids and sediment from Ebro River (NE Spain) and Ebro delta-associated lagoons. Anatoxin-a was detected downstream of the Riba-Roja reservoir with levels ranging from 20 to 1120 ng g-1 dry weight of suspended solids. Okadaic acid was only detected in three samples collected in the Alfacs Bay (Ebro delta, Spain) affected by Dinophysis blooms in 2012.

KW - Cyanotoxins

KW - Ebro

KW - LC-MS/MS

KW - Okadaic acid

KW - Sediment

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JO - Analytical and Bioanalytical Chemistry

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ER -

Liquid chromatography coupled with tandem mass spectrometry to characterise trace levels of cyanobacteria and dinoflagellate toxins in suspended solids and sediments

Abstract

UN SDGs

Keywords

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