Interaction of a multiple antigenic peptide of hepatitis A virus with monolayers and bilayers of acidic, basic, and zwitterionic phospholipids

Y. Cajal, M. A. Alsina, F. Reig, L. Rodriguez, C. Mestres, I. Haro

Research output: Indexed journal article Articlepeer-review

5 Citations (Scopus)


The interaction of the synthetic multiple antigenic peptide construct MAP4-VP1(11-25) with lipid membranes was studied by a combination of spectroscopic and biophysical techniques. MAP4VP1 showed an important surface activity when spread in an air-water interface, indicative of an amphipatic conformation. Since this peptide has a net anionic charge, we studied the degree of interaction with model membranes of zwitterionic dipalmitoylphosphatidylcholine (DPPC), anionic phosphatidylinositol (PI and DPPC/PI 9:1), or cationic stearylamine (SA and DPPC/SA 9:1). MAP4-VP1 was readily soluble in aqueous buffer, yet spontaneously inserted from an aqueous subphase into cationic and zwitterionic monolayers up to limiting pressures of 20-30 mN/m. The induced surface pressure increases were in the order cationic > zwitterionic > anionic monolayers. This indicates that the interaction is mainly driven by electrostatic forces, although there is an important hydrophobic component, responsible for the penetration in monolayers of neutral DPPC. Interaction of MAP4-VP1 with unilamellar vesicles of DPPC, DPPC/SA (9:1), and DPPC/PI (9:1) labeled with 1,6-diphenyl- 1,3,5-hexatriene (DPH) or with 1-anilino-8-naphthalene sulfonic acid (ANS) was determined by changes in fluorescence polarization as a function of temperature. Results showed that the presence of the positively charged SA in the membrane strongly enhances the incorporation of MAP4-VP1 and that the peptide increases the fluidity of the bilayer and decreases the temperature of the phase transition.

Original languageEnglish
Pages (from-to)78-86
Number of pages9
JournalJournal of Colloid and Interface Science
Issue number1
Publication statusPublished - 1 Feb 1998
Externally publishedYes


  • Hepatitis A
  • Langmuir film
  • Lipid- peptide interaction
  • Liposomes
  • Multiple antigenic peptide


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