Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers

C. Mestres; I. Haro; F. Reig; M. A. Alsina

doi:10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N

Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers

C. Mestres^*
, I. Haro
, F. Reig
, M. A. Alsina

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

1 Citation (Scopus)

Abstract

Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.

Original language	English
Pages (from-to)	172-173
Number of pages	2
Journal	Biomedical Chromatography
Volume	11
Issue number	3
DOIs	https://doi.org/10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N
Publication status	Published - May 1997
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Hepatitis A
Liposomes
Monolayers
Phospholipids
Polarization fluorescence

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10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N

Cite this

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title = "Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers",

abstract = "Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.",

keywords = "Hepatitis A, Liposomes, Monolayers, Phospholipids, Polarization fluorescence",

author = "C. Mestres and I. Haro and F. Reig and Alsina, \{M. A.\}",

year = "1997",

month = may,

doi = "10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N",

language = "English",

volume = "11",

pages = "172--173",

journal = "Biomedical Chromatography",

issn = "0269-3879",

publisher = "John Wiley and Sons Ltd",

number = "3",

}

TY - JOUR

T1 - Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers

AU - Mestres, C.

AU - Haro, I.

AU - Reig, F.

AU - Alsina, M. A.

PY - 1997/5

Y1 - 1997/5

N2 - Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.

AB - Myscibility of dipalmitoylphosphatidylcholine(DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipine (CL) and dipalmitoylphosphatidylethanolamine (DPPE) was studied using monomolacular layers of different compositions. The influence of MAP4-VP1 peptide construct related to HAV protein in mixed monolayers of the above cited lipid components was determined following penetration kinetics and compression isotherms. Moreover, liposomes with the same composition as monolayers were saturated with ANS or DPH and incubated with MAP4VP1, and polarization values as well as transition temperatures were determined. In general interaction is maximum with DPPC/DPPG mono and bilayers, followed by DPPC/CL. In these systems electrostatic repulsive forces seem to play a strong role.

KW - Hepatitis A

KW - Liposomes

KW - Monolayers

KW - Phospholipids

KW - Polarization fluorescence

UR - https://www.scopus.com/pages/publications/0031000405

U2 - 10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N

DO - 10.1002/(SICI)1099-0801(199705)11:3<172::AID-BMC680>3.0.CO;2-N

M3 - Article

C2 - 9192112

AN - SCOPUS:0031000405

SN - 0269-3879

VL - 11

SP - 172

EP - 173

JO - Biomedical Chromatography

JF - Biomedical Chromatography

IS - 3

ER -

Insertion of MAP4-VP1 peptide into lipid monolayers and bilayers

Abstract

UN SDGs

Keywords

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