Insect cell culture medium selection and optimisation based on monitoring and economical considerations

M Lecina; A Soley; P Passamani; A Casablancas; J De Gràcia; C Vela; E Espuña; JJ Cairó; F Gòdia

Insect cell culture medium selection and optimisation based on monitoring and economical considerations

M Lecina
, A Soley
, P Passamani
, A Casablancas
, J De Gràcia
, C Vela
, E Espuña
, JJ Cairó
, F Gòdia

Research output: Book chapter › Conference contribution › peer-review

Abstract

The culture of insect cells is currently done using different media specifically developed for these cells (among them, IPL-41, Grace's, and TC-100). The growth of a Sf9 cell line was studied in each of these media, and IPL-41 showed the best results. Nevertheless, this medium contains various carbohydrates as carbon and energy sources, and this fact supposes a complication for the monitoring of the culture. In order to make possible the control of the sugar exhaustion, its formulation was simplified to a unique saccaride source, as it was seen that one of this nutrients was sufficient to enable an efficient growth of the cells.

Original language	English
Title of host publication	Animal Cell Technology Meets Genomics
Editors	F Godia, M Fussenegger
Publisher	Springer Nature
Pages	103-106
Number of pages	4
ISBN (Print)	1-4020-2791-5
Publication status	Published - 2005
Externally published	Yes
Event	18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) - Granada, Spain Duration: 11 May 2003 → 14 May 2003

Conference

Conference	18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT)
Country/Territory	Spain
City	Granada
Period	11/05/03 → 14/05/03

Cite this

@inproceedings{834dd34a351a47279746b148e8af75b7,

title = "Insect cell culture medium selection and optimisation based on monitoring and economical considerations",

abstract = "The culture of insect cells is currently done using different media specifically developed for these cells (among them, IPL-41, Grace's, and TC-100). The growth of a Sf9 cell line was studied in each of these media, and IPL-41 showed the best results. Nevertheless, this medium contains various carbohydrates as carbon and energy sources, and this fact supposes a complication for the monitoring of the culture. In order to make possible the control of the sugar exhaustion, its formulation was simplified to a unique saccaride source, as it was seen that one of this nutrients was sufficient to enable an efficient growth of the cells.",

author = "M Lecina and A Soley and P Passamani and A Casablancas and \{De Gr{\`a}cia\}, J and C Vela and E Espu{\~n}a and JJ Cair{\'o} and F G{\`o}dia",

year = "2005",

language = "English",

isbn = "1-4020-2791-5",

pages = "103--106",

editor = "F Godia and M Fussenegger",

booktitle = "Animal Cell Technology Meets Genomics",

publisher = "Springer Nature",

address = "United Kingdom",

note = "18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT) ; Conference date: 11-05-2003 Through 14-05-2003",

}

Lecina, M, Soley, A, Passamani, P, Casablancas, A, De Gràcia, J, Vela, C, Espuña, E, Cairó, JJ & Gòdia, F 2005, Insect cell culture medium selection and optimisation based on monitoring and economical considerations. in F Godia & M Fussenegger (eds), Animal Cell Technology Meets Genomics. Springer Nature, pp. 103-106, 18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT), Granada, Spain, 11/05/03.

TY - GEN

T1 - Insect cell culture medium selection and optimisation based on monitoring and economical considerations

AU - Lecina, M

AU - Soley, A

AU - Passamani, P

AU - Casablancas, A

AU - De Gràcia, J

AU - Vela, C

AU - Espuña, E

AU - Cairó, JJ

AU - Gòdia, F

PY - 2005

Y1 - 2005

N2 - The culture of insect cells is currently done using different media specifically developed for these cells (among them, IPL-41, Grace's, and TC-100). The growth of a Sf9 cell line was studied in each of these media, and IPL-41 showed the best results. Nevertheless, this medium contains various carbohydrates as carbon and energy sources, and this fact supposes a complication for the monitoring of the culture. In order to make possible the control of the sugar exhaustion, its formulation was simplified to a unique saccaride source, as it was seen that one of this nutrients was sufficient to enable an efficient growth of the cells.

AB - The culture of insect cells is currently done using different media specifically developed for these cells (among them, IPL-41, Grace's, and TC-100). The growth of a Sf9 cell line was studied in each of these media, and IPL-41 showed the best results. Nevertheless, this medium contains various carbohydrates as carbon and energy sources, and this fact supposes a complication for the monitoring of the culture. In order to make possible the control of the sugar exhaustion, its formulation was simplified to a unique saccaride source, as it was seen that one of this nutrients was sufficient to enable an efficient growth of the cells.

UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pure_univeritat_ramon_llull&SrcAuth=WosAPI&KeyUT=WOS:000228864200017&DestLinkType=FullRecord&DestApp=WOS_CPL

M3 - Conference contribution

SN - 1-4020-2791-5

SP - 103

EP - 106

BT - Animal Cell Technology Meets Genomics

A2 - Godia, F

A2 - Fussenegger, M

PB - Springer Nature

T2 - 18th Meeting of the European-Society-of-Animal-Cell-Technology (ESACT)

Y2 - 11 May 2003 through 14 May 2003

ER -

Insect cell culture medium selection and optimisation based on monitoring and economical considerations

Abstract

Conference

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