Influence of phospholipidic charge on the interaction of a multiple antigenic peptide from hepatitis a virus with monolayers and bilayers

C. Mestres; A. Ortiz; I. Haro; F. Reig; M. A. Alsina

doi:10.1021/la960737v

Influence of phospholipidic charge on the interaction of a multiple antigenic peptide from hepatitis a virus with monolayers and bilayers

C. Mestres, A. Ortiz, I. Haro, F. Reig, M. A. Alsina^*

^*Corresponding author for this work

Research output: Indexed journal article › Article › peer-review

10 Citations (Scopus)

Abstract

Miscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipin (CL), and dipalmitoylphosphatidylethanolamine (DPPE) was studied using mono- and bilayers of different compositions. The influence of MAP4-VP1 peptide construct (related to hepatitis A virus protein) in mixed monolayers of the lipids above cited was determined by studying penetration kinetics and compression isotherms. Moreover, liposomes with the same composition than monolayers were saturated with sodium anilinonaphthalene sulfonate or diphenylhexatriene and incubated with MAP4-VP1; polarization values as well as transition temperatures were determined. Results in isotherm and polarization studies showed some degree of interaction between the peptide and the phospholipids assayed. DPPC/ DPPG composition showed the maximum interaction followed by DPPC/CL. The interaction was not strong, suggesting that the entrapment of MAP4-VP1 in liposomes in order to increase its immunogenic activity is possible.

Original language	English
Pages (from-to)	5669-5673
Number of pages	5
Journal	Langmuir
Volume	13
Issue number	21
DOIs	https://doi.org/10.1021/la960737v
Publication status	Published - 15 Oct 1997
Externally published	Yes

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title = "Influence of phospholipidic charge on the interaction of a multiple antigenic peptide from hepatitis a virus with monolayers and bilayers",

abstract = "Miscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipin (CL), and dipalmitoylphosphatidylethanolamine (DPPE) was studied using mono- and bilayers of different compositions. The influence of MAP4-VP1 peptide construct (related to hepatitis A virus protein) in mixed monolayers of the lipids above cited was determined by studying penetration kinetics and compression isotherms. Moreover, liposomes with the same composition than monolayers were saturated with sodium anilinonaphthalene sulfonate or diphenylhexatriene and incubated with MAP4-VP1; polarization values as well as transition temperatures were determined. Results in isotherm and polarization studies showed some degree of interaction between the peptide and the phospholipids assayed. DPPC/ DPPG composition showed the maximum interaction followed by DPPC/CL. The interaction was not strong, suggesting that the entrapment of MAP4-VP1 in liposomes in order to increase its immunogenic activity is possible.",

author = "C. Mestres and A. Ortiz and I. Haro and F. Reig and Alsina, {M. A.}",

year = "1997",

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T1 - Influence of phospholipidic charge on the interaction of a multiple antigenic peptide from hepatitis a virus with monolayers and bilayers

AU - Mestres, C.

AU - Ortiz, A.

AU - Haro, I.

AU - Reig, F.

AU - Alsina, M. A.

PY - 1997/10/15

Y1 - 1997/10/15

N2 - Miscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipin (CL), and dipalmitoylphosphatidylethanolamine (DPPE) was studied using mono- and bilayers of different compositions. The influence of MAP4-VP1 peptide construct (related to hepatitis A virus protein) in mixed monolayers of the lipids above cited was determined by studying penetration kinetics and compression isotherms. Moreover, liposomes with the same composition than monolayers were saturated with sodium anilinonaphthalene sulfonate or diphenylhexatriene and incubated with MAP4-VP1; polarization values as well as transition temperatures were determined. Results in isotherm and polarization studies showed some degree of interaction between the peptide and the phospholipids assayed. DPPC/ DPPG composition showed the maximum interaction followed by DPPC/CL. The interaction was not strong, suggesting that the entrapment of MAP4-VP1 in liposomes in order to increase its immunogenic activity is possible.

AB - Miscibility of dipalmitoylphosphatidylcholine (DPPC) with dipalmitoylphosphatidylglycerol (DPPG), cardiolipin (CL), and dipalmitoylphosphatidylethanolamine (DPPE) was studied using mono- and bilayers of different compositions. The influence of MAP4-VP1 peptide construct (related to hepatitis A virus protein) in mixed monolayers of the lipids above cited was determined by studying penetration kinetics and compression isotherms. Moreover, liposomes with the same composition than monolayers were saturated with sodium anilinonaphthalene sulfonate or diphenylhexatriene and incubated with MAP4-VP1; polarization values as well as transition temperatures were determined. Results in isotherm and polarization studies showed some degree of interaction between the peptide and the phospholipids assayed. DPPC/ DPPG composition showed the maximum interaction followed by DPPC/CL. The interaction was not strong, suggesting that the entrapment of MAP4-VP1 in liposomes in order to increase its immunogenic activity is possible.

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DO - 10.1021/la960737v

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SN - 0743-7463

VL - 13

SP - 5669

EP - 5673

JO - Langmuir

JF - Langmuir

IS - 21

ER -

Influence of phospholipidic charge on the interaction of a multiple antigenic peptide from hepatitis a virus with monolayers and bilayers

Abstract

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